Various lysates were subjected to SDS PAGE followed by western blot with 12497-1-AP (CRTC2,TORC2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of Hela cells, using CRTC2 antibody 12497-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG (green). Blue pseudocolor = DAPI (fluorescent DNA dye).

Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 12497-1-AP (CRTC2,TORC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using KHC1784 (CRTC2 IHC Kit).

Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using KHC1784 (CRTC2 IHC Kit).

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using KHC1784 (CRTC2 IHC Kit).

Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using KHC1784 (CRTC2 IHC Kit).

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using KHC1784 (CRTC2 IHC Kit).

Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using KHC1784 (CRTC2 IHC Kit).

Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using KHC1784 (CRTC2 IHC Kit).

CRTC2,TORC2 fusion protein Ag27167 SDS-PAGE

mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15463-1-AP (MAPKAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human kidney using 15463-1-AP (MAPKAP1 antibody) at dilution of 1:100 (under 10x lens).

Immunohistochemical analysis of paraffin-embedded human kidney using 15463-1-AP (MAPKAP1 antibody) at dilution of 1:100 (under 40x lens).

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15463-1-AP (SIN1 antibody) at dilution of 1:200 (under 40x lens).

IP Result of anti-MAPKAP1 (IP:15463-1-AP, 4ug; Detection:15463-1-AP 1:500) with mouse kidney tissue lysate 4000ug.

Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MAPKAP1 antibody (15463-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

MAPKAP1 fusion protein Ag7753 SDS-PAGE

Various lysates were subjected to SDS PAGE followed by western blot with 27248-1-AP (RICTOR antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27248-1-AP (RICTOR antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27248-1-AP (RICTOR antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 27248-1-AP (RICTOR antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 27248-1-AP (RICTOR antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (-20℃Methanol) fixed NIH/3T3 cells using 27248-1-AP (RICTOR antibody) at dilution of 1:200 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Various lysates were subjected to SDS PAGE followed by western blot with 66867-2-Ig (RICTOR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Non-treated and Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 67778-1-Ig (Phospho-mTOR (Ser2448) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with RICTOR antibody (66867-2-Ig) and mTOR antibody (66888-1-Ig) subsequently.

Non-treated and Rapamycin treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 67778-1-Ig (Phospho-mTOR (Ser2448) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with RICTOR antibody (66867-2-Ig) and mTOR antibody (66888-1-Ig) subsequently.

Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 67778-1-Ig (Phospho-mTOR (Ser2448) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with RICTOR antibody (66867-2-Ig) and mTOR antibody (66888-1-Ig) subsequently.

Non-treated PC-3 and PDGF (HZ-1215) treated PC-3 cells were subjected to SDS PAGE followed by western blot with 29117-1-AP (Phospho-RICTOR (Thr1135) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin antibody as loading control.