Various lysates were subjected to SDS PAGE followed by western blot with 29906-1-AP (TSC1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

mouse kidney tissue were subjected to SDS PAGE followed by western blot with 29906-1-AP (Hamartin/TSC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 29906-1-AP (Hamartin/TSC1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 29906-1-AP (Hamartin/TSC1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 29906-1-AP (Hamartin/TSC1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 29906-1-AP (Hamartin/TSC1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

1X10^6 HeLa cells untreated (dashed lines) or treated with Calyculin A (red) were intracellularly stained with 0.25 ug CoraLite®488 Anti-Human Phospho-Hamartin/TSC1 (Ser511) (CL488-80340, Clone:3N6), or 0.25 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 80% MeOH.

1X10^6 HeLa cells untreated (dashed lines) or treated with Calyculin A (red) were intracellularly stained with 0.5 ug CoraLite®647 Anti-Human Phospho-Hamartin/TSC1 (Ser511) (CL647-80340, Clone:3N6), or 0.5 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 50% MeOH.

1X10^6 HeLa cells untreated (dashed lines) or treated with Calyculin A (red) were intracellularly stained with 0.5 ug CoraLite®555 Anti-Human Phospho-Hamartin/TSC1 (Ser511) (CL555-80340, Clone:3N6) (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with 50% MeOH.

1X10^6 HeLa cells untreated (left) or treated with Calyculin A (right) were intracellularly stained with 0.5 ug CoraLite®555 Anti-Human Phospho-Hamartin/TSC1 (Ser511) (CL555-80340, Clone:3N6) (red), or 0.5 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 50% MeOH.

1X10^6 HeLa cells untreated (dashed lines) or treated with Calyculin A (red) were intracellularly stained with 0.5 ug CoraLite®594 Anti-Human Phospho-Hamartin/TSC1 (Ser511) (CL594-80340, Clone:3N6) (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with 50% MeOH.

Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using KHC1060 (TSC1 IHC Kit).

Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using KHC1060 (TSC1 IHC Kit).

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using KHC1060 (TSC1 IHC Kit).

Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using KHC1060 (TSC1 IHC Kit).

Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using KHC1060 (TSC1 IHC Kit).

Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using KHC1060 (TSC1 IHC Kit).

Non-treated and Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80340-1-RR (Phospho-Hamartin/TSC1 (Ser511) Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Non-treated and Calyculin A treated HeLa and HEK-293 cells were subjected to SDS PAGE followed by western blot with 80340-1-RR (Phospho-Hamartin/TSC1 (Ser511) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

1X10^6 Hela cells untreated (dashed lines) and Calyculin A treated (red) were intracellularly stained with 0.5 ug Anti-Human Phospho-Hamartin/TSC1 (Ser511) (80340-1-RR, Clone:3N6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, or 0.5 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 50% MeOH.

Non-treated and Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80340-1-RR (Phospho-Hamartin/TSC1 (Ser511) Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80340-1-PBS in a different storage buffer formulation.

Non-treated and Calyculin A treated HeLa and HEK-293 cells were subjected to SDS PAGE followed by western blot with 80340-1-RR (Phospho-Hamartin/TSC1 (Ser511) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80340-1-PBS in a different storage buffer formulation.

1X10^6 HeLa cells untreated (dashed lines) and Calyculin A treated (red) were intracellularly stained with 0.5 ug Anti-Human Phospho-Hamartin/TSC1 (Ser511) (80340-1-RR, Clone:3N6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, or 0.5 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 50% MeOH. This data was developed using the same antibody clone with 80340-1-PBS in a different storage buffer formulation.