human urine lysates were subjected to SDS PAGE followed by western blot with 17435-1-AP (Transferrin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 17435-1-AP (Transferrin antibody at dilution of 1:200 (under 10x lens).

Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 17435-1-AP (Transferrin antibody at dilution of 1:200 (under 40x lens).

Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 17435-1-AP (Transferrin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

human plasma were subjected to SDS PAGE followed by western blot with 66171-1-Ig (Transferrin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Transferrin antibody (66171-1-Ig, Clone: 2B4C6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Transferrin antibody (66171-1-Ig, Clone: 2B4C6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66171-1-Ig (Transferrin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66171-1-Ig (Transferrin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite®488 Transferrin antibody (CL488-66171, Clone: 2B4C6 ) at dilution of 1:100.

Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite®488 Transferrin antibody (CL488-66171, Clone: 2B4C6 ) at dilution of 1:100.

Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite®594 Transferrin antibody (CL594-66171, Clone: 2B4C6 ) at dilution of 1:100.

Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite®594 Transferrin antibody (CL594-66171, Clone: 2B4C6 ) at dilution of 1:100.

Standard curve of Human TF ELISA Kit KE00046.

Standard curve of Human TF ELISA Kit KE00046.

Purity of recombinant human Transferrin was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

Recombinant human Transferrin (Cat no: HZ-1317) stimulates dose-dependent proliferation of the OKT4 mouse hybridoma cell line. Cell number was quantitatively assessed by Prestoblue® Cell Viability Reagent. OKT4 cells were treated with increasing concentrations of recombinant Transferrin for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 75-400 ng/mL.
 

Purity of GMP recombinant human Transferrin was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

Recombinant GMP human Transferrin (Cat no: HZ-1317-GMP) stimulates dose-dependent proliferation of the OKT4 mouse hybridoma cell line. Cell number was quantitatively assessed by Prestoblue® Cell Viability Reagent. OKT4 cells were treated with increasing concentrations of recombinant Transferrin for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on the validated bioassay. The EC50 range is 75-400 ng/mL.