IP result of anti-VIRMA/KIAA1429 (IP:25712-1-AP, 4ug; Detection:25712-1-AP 1:600) with MCF-7 cells lysate 800 ug.

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25712-1-AP (VIRMA/KIAA1429 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 25712-1-AP (VIRMA/KIAA1429 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

PC-3 cells were subjected to SDS PAGE followed by western blot with 25712-1-AP (KIAA1429 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 25712-1-AP (VIRMA/KIAA1429 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

1X10^6 MCF-7 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human KIAA1429 (CL488-68235, Clone:3F1A4) (red), or 0.8 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Immunohistochemical analysis of paraffin-embedded human colon tissue slide using KHC0149 (VIRMA/KIAA1429 IHC Kit).

Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using KHC0149 (VIRMA/KIAA1429 IHC Kit).

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using KHC0149 (VIRMA/KIAA1429 IHC Kit).

Various lysates were subjected to SDS PAGE followed by western blot with 68235-1-Ig (KIAA1429 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human KIAA1429 (68235-1-Ig, Clone:3F1A4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Various lysates were subjected to SDS PAGE followed by western blot with 68235-1-Ig (KIAA1429 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 68235-1-Ig (KIAA1429 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68235-1-PBS in a different storage buffer formulation.

1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human KIAA1429 (68235-1-Ig, Clone:3F1A4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68235-1-PBS in a different storage buffer formulation.

Various lysates were subjected to SDS PAGE followed by western blot with 68235-1-Ig (KIAA1429 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68235-1-PBS in a different storage buffer formulation.