WB result of YTHDF3-specific antibody (25537-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-YTHDF3-specific transfected HeLa cells.
Various lysates were subjected to SDS PAGE followed by western blot with 25537-1-AP (YTHDF3-specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25537-1-AP (YTHDF3-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25537-1-AP (YTHDF3-specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using YTHDF3-specific antibody (25537-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
