mouse kidney tissue lysates were subjected to SDS PAGE followed by western blot with 21115-1-AP (ACE2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
1X10^6 NIH/3T3 cells were stained with 0.20 ug/test Anti-Human ACE2 (21115-1-AP) (red) or 0.20 ug control antibody (blue) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000. Fixed with90% MeOH.
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21115-1-AP (ACE2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21115-1-AP (ACE2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using ACE2 antibody (21115-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), PTPRO antibody (67000-1-Ig, Clone: 2F2B4, red).
Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using ACE2 antibody (21115-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), PTPRO antibody (67000-1-Ig, Clone: 2F2B4, red).
IP Result of anti-ACE2 (IP:21115-1-AP, 5ug; Detection:21115-1-AP 1:1000) with mouse kidney tissue lysate 4000ug.