Actin visualization in HeLa, PC3, U2OS, MEF, LNCaP, and BHK cells using Actin-Chromobody: Fluorescent microscopy images of cells transfected with Actin-Chromobody; green: TagGFP2, red: TagRFP (Evrogen), and blue: DAPI counterstain.
Nuclear Actin-Chromobody: NIH 3T3 cells (murine fibroblasts) transfected with the nuclear Actin-Chromobody (TagGFP2, green) and the Lamin-Chromobody (TagRFP, red) were starved in serum-free medium for 24 hours. Nuclear Actin-Chromobody probed endogenous F-actin assembly and distribution is shown 30 s after stimulation with serum. Courtesy of Matthias Plessner.
The time lapse analysis reveals the reorganization of actin after treatment with Cytochalasin D: HeLa cells were subjected to confocal imaging upon treatment with 2 µM of Cytochalasin D for 1 hour and recovery for 4 hours. Actin Chromobody enables monitoring of actin dynamics in real-time in living cells.