Various lysates were subjected to SDS PAGE followed by western blot with 20597-1-AP (CD9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20597-1-AP (CD9 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20597-1-AP (CD9 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human CD9 (20597-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CD9 antibody (20597-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Various lysates were subjected to SDS PAGE followed by western blot with 66866-1-Ig (CD81 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66866-1-Ig (CD81 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66866-1-Ig (CD81 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD81 antibody (66866-1-Ig, Clone: 1G2C6 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD81 antibody (66866-1-Ig, Clone: 1G2C6 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

1X10^6 Jurkat cells were surface stained with 0.4 ug Anti-Human CD81 (66866-1-Ig, Clone: 1G2C6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were not fixed.

1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human CD107a / LAMP1 (65051-1-Ig, Clone:H4A3) and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 65051-1-Ig (CD107a antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

1X10^6 HeLa cells were intracellularly stained with 5 ul CoraLite® Plus 647 Anti-Human CD107a (CL647-65051, Clone:H4A3) (red), or Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 647-conjugated CD107a antibody (CL647-65051, Clone: H4A3) at dilution of 1:400. F-actin was stained using CL488-phalloidin (green) and DNA was stained by DAPI (blue).

1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human CD107b / LAMP2 (65053-1-Ig, Clone:H4B4) and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or Mouse IgG1 Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Intracellular Staining Permeabilization Wash Buffer.

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 65053-1-Ig (CD107b antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

1X10^6 HeLa cells were intracellularly stained with 5 ul CoraLite®488 Anti-Human CD107a (CL488-65051, Clone:H4A3) (red), or Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®488-conjugated CD81 antibody (CL488-66866, Clone: 1G2C6 ) at dilution of 1:200.

Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®488-conjugated CD81 antibody (CL488-66866, Clone: 1G2C6 ) at dilution of 1:200.

1X10^6 Jurkat cells were stained with 0.2 ug CoraLite®488-conjugated Anti-Human CD81 (CL488-66866, Clone: 1G2C6) (green), or stained with 0.2 ug isotype control (black). Cells were fixed with 90% MeOH.

1X10^6 Jurkat cells were intracellularly stained with 5 ul CoraLite® Plus 647 Anti-Human CD107b (CL647-65053, Clone:H4B4) (red) or Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 647-conjugated CD107b antibody (CL647-65053, Clone: H4B4) at dilution of 1:50 and CoraLite®488-conjugated CD107a antibody (CL488-65051, Clone: H4A3, green) at dilution of 1:50.