Raji cells were subjected to SDS PAGE followed by western blot with 66298-1-Ig (CD19 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Untreated and PNGase F-treated lysates of Raji cells were subjected to SDS PAGE followed by western blot with 66298-1-Ig (CD19 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808).

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66298-1-Ig (CD19 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66298-1-Ig (CD19 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

1X10^6 Raji cells were stained with 0.20ug CD19 antibody (66298-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH.

Daudi cells were subjected to SDS PAGE followed by western blot with 27949-1-AP (CD19 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27949-1-AP (CD19 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27949-1-AP (CD19 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD19 antibody (27949-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

1X10^6 human PBMCs were surface co-stained with Anti-Human CD3 and 5 ul FITC Plus Anti-Human CD19 (FITC-65197, Clone:4G7), or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

1X10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD19 (FITC-65197, Clone:4G7), or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

1X10^6 human peripheral blood lymphocytes were surface stained with 0.25 ug Anti-Human CD19 (65197-1-Ig, Clone: 4G7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), or stained with 0.25 ug Mouse IgG1 Isotype Control and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (black). Cells were not fixed.

1X10^6 human peripheral blood lymphocytes were surface stained with 0.5 ug Anti-Human CD19 (65110-1-Ig, Clone: HIB19) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), or stained with 0.5 ug isotype control and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (black). Cells were not fixed.

1X10^6 human peripheral blood lymphocytes were surface stained with 0.125 ug APC-anti-human CD19 (APC-65110, clone HIB19) (red) or 0.125 ug APC-mouse IgG1 isotype control (blue). Cells were not fixed.

1X10^6 human PBMCs were surface co-stained with PE Anti-Human CD3 and 5 ul Atlantic Blue™ Anti-Human CD19 (AB-65197, Clone: 4G7) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

1X10^6 human PBMCs were surface stained with 5 ul Atlantic Blue™ Anti-Human CD19 (AB-65197, Clone:4G7) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

1X10^6 human peripheral blood lymphocytes were surface stained with APC-Anti-Human CD3 (APC-65151, Clone: UCHT1) and 5 ul CoraLite®488-conjugated Anti-Human CD19 (CL488-65197, Clone: 4G7). Cells were not fixed.

1X10^6 human peripheral blood lymphocytes were surface stained with APC-Anti-Human CD3 (APC-65151, Clone: UCHT1) and CoraLite®488-conjugated Mouse IgG1 isotype control. Cells were not fixed.