MCF-7 cells were subjected to SDS PAGE followed by western blot with 18330-1-AP (CD24 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

---

Various lysates were subjected to SDS PAGE followed by western blot with 10600-1-AP (CD24 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

---

1X10^6 BALB/c mouse splenocytes were surface stained with 0.5 ug Anti-Mouse CD24 (65121-1-Ig, Clone: M1/69) and FITC anti-rat IgG2b Antibody at dilution 1:200 (green), or stained with 0.5 ug isotype control and FITC anti-rat IgG2b Antibody at dilution 1:200 (black). Cells were not fixed.

---

1X10^6 mouse splenocytes were surface stained with FITC Anti-Mouse CD3 (FITC-65077, Clone: 17A2) and 0.06 ug APC-Rat IgG2b isotype control (left) or 0.06 ug APC Anti-Mouse CD24 (APC-65121, Clone: M1/69) (right). Cells were not fixed.

---

Various lysates were subjected to SDS PAGE followed by western blot with 67627-1-Ig (CD24 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

---

1X10^6 Ramos cells were stained with 0.2 ug Anti-Human CD24 (67627-1-Ig, Clone: 1H5C4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), or stained with 0.2 ug isotype control antibody and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (black). Cells were fixed with 90% MeOH.

---

1X10^6 mouse splenocytes were surface stained with 5 ul CoraLite® Plus 647 Anti-Mouse CD3 (17A2) (CL647-65077, Clone: 17A2) and CoraLite® Plus 488-conjugated rat IgG2b isotype control (left) or 5 ul CoraLite® Plus 488 Anti-Mouse CD24 (CL488-65121, Clone: M1/69) (right). Cells were not fixed.

---

1X10^6 C57BL/6 mouse splenocytes were surface stained with FITC-Anti-Mouse CD3 (FITC-65077, Clone: 17A2) and 5.00 ul CoraLite®647-conjugated Anti-Mouse CD24 (CL647-65121, Clone: M1/69). Cells were not fixed.

---

1X10^6 C57BL/6 mouse splenocytes were surface stained with FITC-Anti-Mouse CD3 (FITC-65077, Clone: 17A2) and CoraLite®647-conjugated rat IgG2b isotype control antibody. Cells were not fixed.

---

1X10^6 Ramos cells were stained with 0.4 ug CoraLite®594 Anti-Human CD24 (CL594-67627, Clone:1H5C4) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL594-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---