Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using KHC0030 (CD44 IHC Kit).

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using KHC0030 (CD44 IHC Kit). This view is captured from cancer adjacent normal tissue.

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using KHC0030 (CD44 IHC Kit).

WB result of CD44 antibody (15675-1-AP, 1:6000) with si-Control and si-CD44 transfected HeLa cells.

MDA-MB-231 (CD44+), HeLa (CD44+), A549 (CD44+), MCF-7, Jurkat cell lysates were subjected to SDS PAGE followed by western blot with 15675-1-AP (CD44 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 15675-1-AP (CD44 antibody) at dilution of 1:200 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15675-1-AP (CD44 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15675-1-AP (CD44 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

IP Result of anti-CD44 (IP:15675-1-AP, 4ug; Detection:15675-1-AP 1:2000) with HeLa cells lysate 2400ug.

1X10^6 HeLa cells were stained with 0.20ug CD44 antibody (15675-1-AP, red) and control antibody (blue). Fixed with 90% MeOH.

WB result of CD44 antibody (60224-1-Ig, 1:4000) with si-Control and si-CD44 transfected HeLa cells.

HeLa (CD44+), A549 (CD44+), MDA-MB-231 (CD44+), MCF-7, Jurkat cell lysates were subjected to SDS PAGE followed by western blot with 60224-1-Ig (CD44 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using 60224-1-Ig (CD44 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using 60224-1-Ig (CD44 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

1X10^6 Raji cells were stained with 0.2ug CD44 antibody (60224-1-Ig, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). FITC-Goat anti-mouse IgG with dilution 1:1000.

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60224-1-Ig (CD44 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60224-1-Ig (CD44 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

1X10^6 human peripheral blood lymphocytes were surface stained with 0.25 ug Anti-Human CD44 (65063-1-Ig, Clone:F10-44-2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), or stained with 0.25 ug Mouse IgG2a Isotype Control Antibody and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (black). Cells were not fixed.

1X10^6 mouse splenocytes were surface stained with 5 ul CoraLite® Plus 647 Anti-Mouse CD3 (17A2) (CL647-65077, Clone: 17A2) and 0.25 ug FITC-Rat IgG2b isotype control (left) or 0.25 ug FITC Anti-Mouse CD44 (FITC-65117, Clone: IM7) (right). Cells were not fixed.

1X10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD44 (FITC-65063, Clone: F10-44-2), or Mouse IgG2a Isotype Control. Cells were not fixed. Lymphocytes were gated.

1X10^6 human PBMCs were surface stained with 5 ul PE Anti-Human CD44 (PE-65063, Clone:F10-44-2) or Mouse IgG2a Isotype Control. Cells were not fixed. Lymphocytes were gated.

1X10^6 human PBMCs were surface stained with 5 ul PE Anti-Human CD44 (PE-65063, Clone:F10-44-2) or Mouse IgG2a Isotype Control. Cells were not fixed. Lymphocytes were gated.

1X10^6 mouse splenocytes were surface stained with 5 ul CoraLite® Plus 647 Anti-Mouse CD3 (CL647-65077, Clone: 17A2), and 0.5 ug Anti-Mouse CD44 (65117-1-Ig, Clone: IM7) and FITC anti-rat IgG2b Antibody at dilution 1:200. Cells were not fixed.

1X10^6 mouse splenocytes were surface stained with 5 ul CoraLite® Plus 647 Anti-Mouse CD3 (CL647-65077, Clone: 17A2), and 0.5 ug Rat IgG2b isotype control and FITC anti-rat IgG2b Antibody at dilution 1:200. Cells were not fixed.