Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-HSP60 (66041-1-Ig) labeled with FlexAble CoraLite® Plus 405 Kit (KFA026, cyan). Cell nuclei are in red. Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with 0.5 µg anti-human CD45 antibody (clone HI30, 65109-1-Ig) labeled with FlexAble CoraLite® Plus 405 Kit (KFA026).

Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with 0.5 µg anti-human CD3 antibody (clone UCHT1, 65151-1-Ig) labeled with FlexAble CoraLite® Plus 405 Kit (KFA026).

Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with 0.5 µg anti-human CD4 antibody (clone RPA-T4, 65143-1-Ig) labeled with FlexAble CoraLite® Plus 405 Kit (KFA026).

FlexAble Workflow

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-TOM70 (14528-1-AP) labeled with FlexAble CoraLite® Plus 405 Kit (KFA006, cyan). Cell nuclei are in red. Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were intracellularly stained with 0.5 µg anti-human P62 (SQSTM1) antibody (18420-1-AP) labeled with FlexAble CoraLite® Plus 405 Kit (KFA006, cyan) or with 0.5 µg isotype control antibody labeled with FlexAble CoraLite® Plus 405 Kit (KFA006, red).

Flow cytometry of HEK293. 1X10^6 HEK293 cells were intracellularly stained with 0.5 µg anti-human P62 (SQSTM1) antibody (18420-1-AP) labeled with FlexAble CoraLite® Plus 405 Kit (KFA006, blue) or with 0.5 µg isotype control antibody labeled with FlexAble CoraLite® Plus 405 Kit (KFA006, red) or with FlexLinker CoraLite® Plus 405 for rabbit IgG (orange).  Unstained HEK293 cells were used as a reference (red). 

FlexAble Workflow