Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-HSP60 (66041-1-Ig) labeled with FlexAble CoraLite® Plus 405 Kit (KFA026, cyan). Cell nuclei are in red. Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with 0.5 µg anti-human CD45 antibody (clone HI30, 65109-1-Ig) labeled with FlexAble CoraLite® Plus 405 Kit (KFA026).

Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with 0.5 µg anti-human CD3 antibody (clone UCHT1, 65151-1-Ig) labeled with FlexAble CoraLite® Plus 405 Kit (KFA026).

Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with 0.5 µg anti-human CD4 antibody (clone RPA-T4, 65143-1-Ig) labeled with FlexAble CoraLite® Plus 405 Kit (KFA026).

FlexAble Workflow

PFA-fixed HeLa cells were stained with anti-GNL3 (67169-1-Ig) labeled with FlexAble CoraLite® Plus 405 Kit (KFA046, cyan) and anti-Tubulin (66240-1-Ig) labeled with FlexAble CoraLite® Plus 750 Kit (KFA044, grey). Epifluorescence images were acquired with a 20x objective and post-processed.

PFA-fixed HeLa cells were stained with anti-Actin labeled with FlexAble CoraLite® Plus 405 Kit (KFA046, cyan) and Sytox Deep Red (cell nuclei, magenta). Epifluorescence images were acquired with a 20x objective and post-processed.

Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD45 (clone HI30, 65109-1-Ig) labeled with FlexAble CoraLite® Plus 750 Kit (KFA024) and anti-human CD3 (clone OKT3, 65133-1-Ig) labeled with FlexAble CoraLite® Plus 405 Kit (KFA046).

Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD3 (clone UCHT1, 65151-1-Ig) labeled with FlexAble CoraLite® Plus 750 Kit (KFA024) and anti-human CD8 (clone UCHT4, 65204-1-Ig) labeled with FlexAble CoraLite® Plus 405 Kit (KFA046).

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-Tubulin antibody labeled with FlexAble CoraLite® Plus 405 Kit (KFA066, cyan) and anti-HDAC2 (67165-1-Ig) labeled with FlexAble CoraLite® Plus 750 Kit (KFA064, red). Epifluorescence images were acquired with a 20x objective and post-processed.

Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD3 (clone UCHT1, 65151-1-Ig) labeled with FlexAble CoraLite Plus 555 Kit (KFA022) and anti-human CD4 (clone OKT4, 65134-1-Ig) labeled with FlexAble CoraLite® Plus 405 Kit (KFA066).  Cells are not fixed, lymphocytes are gated. 

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-TOM70 (14528-1-AP) labeled with FlexAble CoraLite® Plus 405 Kit (KFA006, cyan). Cell nuclei are in red. Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were intracellularly stained with 0.5 µg anti-human P62 (SQSTM1) antibody (18420-1-AP) labeled with FlexAble CoraLite® Plus 405 Kit (KFA006, cyan) or with 0.5 µg isotype control antibody labeled with FlexAble CoraLite® Plus 405 Kit (KFA006, red).

Flow cytometry of HEK293. 1X10^6 HEK293 cells were intracellularly stained with 0.5 µg anti-human P62 (SQSTM1) antibody (18420-1-AP) labeled with FlexAble CoraLite® Plus 405 Kit (KFA006, blue) or with 0.5 µg isotype control antibody labeled with FlexAble CoraLite® Plus 405 Kit (KFA006, red) or with FlexLinker CoraLite® Plus 405 for rabbit IgG (orange).  Unstained HEK293 cells were used as a reference (red). 

FlexAble Workflow

Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD20 (Human IgG1) labeled with FlexAble CoraLite® Plus 405 Kit (KFA108) and anti-human CD19 (Mouse IgG1, clone 4G7) conjugated with PE  (PE-65197).