Various lysates were subjected to SDS PAGE followed by western blot with 17168-1-AP (Histone-H3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

1X10^6 HeLa cells were stained with 0.20ug Histone-H3 antibody (17168-1-AP, red) and control antibody (blue). Fixed with 90% MeOH.

Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 17168-1-AP (Histone-H3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 17168-1-AP (Histone-H3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

IP result of anti-Histone-H3 (IP:17168-1-AP, 4ug; Detection:17168-1-AP 1:8000) with MCF-7 cells lysate 2120 ug.

Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Histone-H3 antibody (17168-1-AP) at dilution of 1:1200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Beta Actin antibody (66009-1-Ig, Clone: 2D4H5, red).

HeLa cells were subjected to SDS PAGE followed by western blot with 16047-1-AP (Histone H4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Chromatin was prepared from HEK-293 cells. The ChIP was performed with 10 µg of cross-linked chromatin, 5 µg of 16047-1-AP or Normal Rabbit IgG, and 60µl of Protein A sepharose beads. The immunoprecipitated DNA was quantified by real time PCR. Primers are located in the first kb of the transcribed region.

Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 16047-1-AP (Histone H4 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

IP result of anti-Histone H4 (IP:16047-1-AP, 3ug; Detection:16047-1-AP 1:600) with HeLa cells lysate 2000ug.

Immunofluorescent analysis of HeLa cells using 16047-1-AP (Histone H4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Histone H4 (16047-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

WB result of Histone H1.2 antibody (19649-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-Histone H1.2 transfected MCF-7 cells.

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Histone H1.2 antibody (19649-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

L02 cells were subjected to SDS PAGE followed by western blot with 19649-1-AP (HIST1H1C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human ovary tumor using 19649-1-AP (HIST1H1C antibody) at dilution of 1:100 (under 40x lens).

Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Histone H1.2 antibody (19649-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

IP Result of anti-Histone H1.2 (IP:19649-1-AP, 3ug; Detection:19649-1-AP 1:500) with HeLa cells lysate 1600ug.

Various lysates were subjected to SDS PAGE followed by western blot with 15857-1-AP (Histone H2B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Mouse tumour sections were labelled with H2B (15857-1-AP, red), Dendra2 (green), and DAPI. The image was credited by Dr. Hae Lin Jang from Harvard Medical School.

Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15857-1-AP (Histone H2B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15857-1-AP (Histone H2B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

IP Result of anti-Histone H2B (IP:15857-1-AP, 4ug; Detection:15857-1-AP 1:500) with HEK-293 cells lysate 2000ug.

Various lysates were subjected to SDS PAGE followed by western blot with 66863-1-Ig (Phospho-Histone H3 (Ser10) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 66863-1-Ig (PHH3 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Phospho-Histone H3 (Ser10) antibody (66863-1-Ig, Clone: 4C7G2 ) at dilution of 1:1500 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), Alpha Tubulin antibody (11224-1-AP, green).

1X10^6 HepG2 cells were intracellularly stained with 0.5 ug Anti-Human Phospho-Histone H3 (Ser10) (66863-1-Ig, Clone:4C7G2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.5 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (black). Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100.

Non-treated and Calyculin A treated cells were subjected to SDS PAGE followed by western blot with 66863-1-Ig (Phospho-Histone H3 (Ser10) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

Immunohistochemical analysis of paraffin-embedded Jurkat cells slide using 66863-1-Ig (Phospho-Histone H3 (Ser10) antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded Jurkat cells slide using 66863-1-Ig (Phospho-Histone H3 (Ser10) antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded Jurkat cells slide using 66863-1-Ig (Phospho-Histone H3 (Ser10) antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded Jurkat cells slide using 66863-1-Ig (Phospho-Histone H3 (Ser10) antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 66863-1-Ig (Phospho-Histone H3 (Ser10) antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 66863-1-Ig (Phospho-Histone H3 (Ser10) antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66863-1-Ig (Phospho-Histone H3 (Ser10) antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66863-1-Ig (Phospho-Histone H3 (Ser10) antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 66863-1-Ig (Phospho-Histone H3 (Ser10) antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 66863-1-Ig (Phospho-Histone H3 (Ser10) antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66863-1-Ig (Phospho-Histone H3 (Ser10) antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66863-1-Ig (Phospho-Histone H3 (Ser10) antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Phospho-Histone H3 (Ser10) antibody (66863-1-Ig, Clone: 4C7G2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Phospho-Histone H3 (Ser10) antibody (66863-1-Ig, Clone: 4C7G2 ) at dilution of 1:1500 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), Alpha Tubulin antibody (11224-1-AP, green).

Immunofluorescent analysis of (4% PFA) fixed A549 cells using Phospho-Histone H3 (Ser10) antibody (66863-1-Ig, Clone: 4C7G2 ) at dilution of 1:1500 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), Alpha Tubulin antibody (11224-1-AP, green).

Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Phospho-Histone H3 (Ser10) antibody (66863-1-Ig, Clone: 4C7G2 ) at dilution of 1:1500 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), Alpha Tubulin antibody (11224-1-AP, green).

Immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using Phospho-Histone H3 (Ser10) antibody (66863-1-Ig, Clone: 4C7G2 ) at dilution of 1:1500 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), Alpha Tubulin antibody (11224-1-AP, green).

Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using Phospho-Histone H3 (Ser10) antibody (66863-1-Ig, Clone: 4C7G2 ) at dilution of 1:1200 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), Alpha Tubulin antibody (11224-1-AP, green).

Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using Phospho-Histone H3 (Ser10) antibody (66863-1-Ig, Clone: 4C7G2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

1X10^6 nocodazole treated HeLa cells were intracellularly stained with 0.25 ug Anti-Human Phospho-Histone H3 (Ser10) (66863-1-Ig, Clone:4C7G2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.25 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Various lysates were subjected to SDS PAGE followed by western blot with 17510-1-AP (Histone H1.0 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Histone H1.0 antibody (17510-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

IP Result of anti-Histone H1.0 (IP:17510-1-AP, 4ug; Detection:17510-1-AP 1:500) with A431 cells lysate 2400ug.

human brain tissue were subjected to SDS PAGE followed by western blot with 16441-1-AP (H2AFZ antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16441-1-AP (Histone H2A.z Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 16441-1-AP (Histone H2A.z antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

HepG2 cells were subjected to SDS PAGE followed by western blot with 17030-1-AP (H2AFY2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

IP Result of anti-H2AFY2 (IP:17030-1-AP, 4ug; Detection:17030-1-AP 1:500) with HepG2 cells lysate 1000ug.