The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.
The activity was determined by the dose-dependent inhibition of IL-4 induced proliferation of mouse HT-2 cells (BALB/c spleen activated by sheep erythrocytes in the presence of IL-2) using Promega CellTiter96® Aqueous Non-Radioactive Cell Proliferation Assay.
The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.
Recombinant human BMP-4 (HZ-1045) stimulates dose-dependent induction of alkaline phosphatase production in the ATDC-5 mouse chondrogenic cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. ATDC-5 cells were treated with increasing concentrations of recombinant human BMP-4 for 72 hrs hours before lysis and addition of pNPP. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 values range from 1.5-9 ng/ml.
The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.
Recombinant human BMP-2 (HZ-1128) stimulates dose-dependent induction of alkaline phosphatase production in the ATDC-5 mouse chondrogenic cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. ATDC-5 cells were treated with increasing concentrations of recombinant human BMP-2 for 72 hours before lysis and addition of pNPP. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 values range from 7.5-37.5 ng/ml.
The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.
Recombinant human FGFbasic-TS (HZ-1285) stimulates dose-dependent proliferation of the HDFa human primary fibroblast cell line. Cell number was quantitatively assessed by Promega CellTiter 96® cell viability reagent. HDFa cells were treated with increasing concentrations of recombinant FGFbasic-TS for 48 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.05-0.3 ng/mL.
The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.
The activity was determined by the dose-dependent stimulation of the proliferation of 7TD1 cells (mouse hybridoma cell line) using CellTiter 96® AQueous One Solution Cell Proliferation Assay.
The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.
Recombinant human Activin A (HZ-1138) causes dose-dependent inhibition of proliferation in the Mouse plasmacytoma (MPC-11) cell line. Proliferation of the MPC-11 cell line was assessed using Promega CellTiter 96®. MPC-11 cells were treated with increasing concentrations of recombinant human Activin A for 72 hrs hours before addition of CellTiter96® reagent. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 values range from 0.5-3.5 ng/ml.
The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.
The activity was determined by the dose-dependent stimulation of the proliferation of mouse D10S cells using Promega CellTiter96® Aqueous Non-Radioactive Cell Proliferation Assay.
The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.
Recombinant human IL-4 (HZ-1004) stimulates dose-dependent proliferation of the TF-1 human erythroleukemic indicator cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. TF-1 cells were treated with increasing concentrations of recombinant IL-4 for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.07-0.4 ng/mL.
