IP sample detected with different secondary antibodies. Normal Rabbit IgG (30000-0-AP) as control.

IP sample detected with different secondary antibodies. Normal Rabbit IgG (30000-0-AP) as control.

1X10^6 Jurkat cells were intracellularly stained with 0.2 ug Anti-Rabbit IgG control (30000-0-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

1X10^6 Jurkat cells were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-KI67 (CL488-27309) (red),and 0.2 ug Control CoraLite® Plus 488 Rabbit IgG (CL488-30000) (Solid line). Blank (Dotted line). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 647-conjugated Rabbit IgG control (CL647-30000) (blue, solid line) or CoraLite® Plus 647 Anti-IGF2R-Specific (CL647-20253) (red), or not stained (blank) (blue, dashed line). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

1X10^6 PC-3 cells untreated (dashed lines) or treated with Calyculin A (red) were intracellularly stained with 0.13 ug CoraLite®555 Anti-Phospho-STK11/LKB1 (Thr189) Rabbit Recombinant antibody (CL555-80127), or 0.13 ug CoraLite®555 Rabbit IgG control (CL555-30000) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594-conjugated Rabbit IgG control (CL594-30000 (blue, solid line) or 0.4 ug CoraLite®594 Anti-Galectin-3 (CL594-14979) (red), or not stained (blank) (blue, dashed line). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

1X10^6 HeLa cells were intracellularly stained with 0.5 ug FITC Anti-Rabbit IgG control (FITC-30000) (red), or 0.5 ug FITC Anti-Human ATF4 (FITC-10835) (green), or unstained (blue). Cells were fixed with 4% PFA and permeabilized with Intracellular Staining Permeabilization Wash Buffer.

1X10^6 K-562 cells were stained with 0.20ug Mouse IgG1 isotype control antibody (66360-1-Ig, red) and another control antibody (blue). Fixed with 90% MeOH.

Various lysates were subjected to SDS PAGE followed by western blot with 66360-1-Ig (Mouse IgG1 isotype control antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 66360-1-Ig (Mouse IgG1 isotype control antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Various lysates (50 ug/lane) were subjected to SDS PAGE followed by western blot with 66360-3-Ig (Mouse IgG2b isotype control antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

1X10^6 Jurkat cells were stained with 0.20ug Mouse IgG2b isotype control antibody (66360-3-Ig, red) or control antibody (blue). Fixed with 90% MeOH.