Co-immunoprecipitation (Co-IP) troubleshooting helps identify why co-immunoprecipitation experiments fail to pull down the bait protein, recover the prey protein, or distinguish specific interactions from non-specific binding. Common problems often come from missing controls, unsuitable lysis or wash conditions, disrupted protein interactions, or poor prey solubility.

In this video, we walk through common Co-IP troubleshooting scenarios and share practical tips to improve pulldown specificity, preserve protein interactions, and generate more reliable interaction data.