Immunoprecipitation (IP) troubleshooting helps identify why immunoprecipitation experiments produce high background, poor pulldown, weak signal, or low protein recovery. Common problems can result from missing controls, unsuitable lysis or wash conditions, incorrect antibody or bead selection, or inefficient elution. In this video, we walk through common IP troubleshooting scenarios and share practical tips to improve specificity, signal strength, and target protein recovery.