|Positive WB detected in||HEK-293 cells, PC-3 cells, HeLa cells, HepG2 cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human skeletal muscle tissue, human kidney tissue, human renal cell carcinoma tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:1000-1:8000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:100-1:400|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
The immunogen of 21923-1-AP is ACC1 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, rat|
|Cited Reactivity||human, pig, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ACC1 fusion protein Ag16452|
|Full Name||acetyl-Coenzyme A carboxylase alpha|
|Calculated molecular weight||2383 aa, 275 kDa|
|Observed molecular weight||260-270 kDa|
|GenBank accession number||BC137287|
|Gene ID (NCBI)||31|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
ACACA(Acetyl-CoA carboxylase 1, ACC), also named as ACAC, ACC1 and ACCA, belongs to the biotin containing enzyme family. It catalyzes the synthesis of malonyl-CoA, which is an intermediate substrate playing a pivotal role in the regulation of fatty acid metabolism and energy production. ACACA is involved in the biosynthesis of fatty acids, and malonyl-CoA produced is used as a building block to extend the chain length of fatty acids by fatty acid synthase (FAS)(PMID:19900410). It has 4 isoforms produced by alternative promoter usage with the molecular weight between 260 kDa and 270 kDa.
|Product Specific Protocols|
|WB protocol for ACC1 antibody 21923-1-AP||Download protocol|
|IHC protocol for ACC1 antibody 21923-1-AP||Download protocol|
|IF protocol for ACC1 antibody 21923-1-AP||Download protocol|
|IP protocol for ACC1 antibody 21923-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Kishor (Verified Customer) (02-22-2019)
I have got some good results with this Ab, but the band are not at the exact place (molecular weight) where it supposed to be