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  • KD/KO Validated

ACC1 Polyclonal antibody

ACC1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 21923-1-AP

Synonyms

ACAC, ACACA, ACC, ACC1, ACCA, Acetyl CoA carboxylase 1



Tested Applications

Positive WB detected inHEK-293 cells, PC-3 cells, HeLa cells, HepG2 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman skeletal muscle tissue, human kidney tissue, human renal cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21923-1-AP is ACC1 Fusion Protein expressed in E. coli.

Tested Reactivity human, rat
Cited Reactivity human, pig, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACC1 fusion protein Ag16452
Full Name acetyl-Coenzyme A carboxylase alpha
Calculated molecular weight 2383 aa, 275 kDa
Observed molecular weight 260-270 kDa
GenBank accession numberBC137287
Gene symbol ACACA
Gene ID (NCBI) 31
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ACACA(Acetyl-CoA carboxylase 1, ACC), also named as ACAC, ACC1 and ACCA, belongs to the biotin containing enzyme family. It catalyzes the synthesis of malonyl-CoA, which is an intermediate substrate playing a pivotal role in the regulation of fatty acid metabolism and energy production. ACACA is involved in the biosynthesis of fatty acids, and malonyl-CoA produced is used as a building block to extend the chain length of fatty acids by fatty acid synthase (FAS)(PMID:19900410). It has 4 isoforms produced by alternative promoter usage with the molecular weight between 260 kDa and 270 kDa.

Protocols

Product Specific Protocols
WB protocol for ACC1 antibody 21923-1-APDownload protocol
IHC protocol for ACC1 antibody 21923-1-APDownload protocol
IF protocol for ACC1 antibody 21923-1-APDownload protocol
IP protocol for ACC1 antibody 21923-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Bioconjug Chem

Genetically-encoded FapR-NLuc as a biosensor to determine malonyl-CoA in situ at subcellular scales.

Authors - Yipeng Du
  • KD Validated
mouseWB

Biochem Biophys Res Commun

microRNA-16-5p promotes 3T3-L1 adipocyte differentiation through regulating EPT1.

Authors - Jingjing Xu
humanWB,IHC

Onco Targets Ther

MEX3C regulates lipid metabolism to promote bladder tumorigenesis through JNK pathway.

Authors - Haichao Chao
WB

Animals (Basel)

Data Mining and Validation of AMPK Pathway as a Novel Candidate Role Affecting Intramuscular Fat Content in Pigs.

Authors - Chaogang Yao
humanWB

Oncotarget

PMID: 28052008

Authors - Chaojun Xue
pigWB

Biochim Biophys Acta

Gestational dietary betaine supplementation suppresses hepatic expression of lipogenic genes in neonatal piglets through epigenetic and glucocorticoid receptor-dependent mechanisms.

Authors - Demin Cai

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Kishor (Verified Customer) (02-22-2019)

I have got some good results with this Ab, but the band are not at the exact place (molecular weight) where it supposed to be

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
ACC1 Antibody Western Blot, validation (1:1000 dilution) in (Cat no:21923-1-AP)