ACAT2 Polyclonal antibody

ACAT2 Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, FC, ELISA

Conjugate

Unconjugated

Cat no : 14755-1-AP

Synonyms

ACAT2, ACTL



Tested Applications

Positive WB detected inmouse liver tissue, L02 cells, Caco-2 cells, HepG2 cells
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman liver tissue, human lung tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 4 publications below

Product Information

14755-1-AP targets ACAT2 in WB, IP, IHC, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACAT2 fusion protein Ag6503
Full Name acetyl-Coenzyme A acetyltransferase 2
Calculated molecular weight 41 kDa
Observed molecular weight 40-42 kDa
GenBank accession numberBC000408
Gene symbol ACAT2
Gene ID (NCBI) 39
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ACAT2 antibody 14755-1-APDownload protocol
IHC protocol for ACAT2 antibody 14755-1-APDownload protocol
IP protocol for ACAT2 antibody 14755-1-APDownload protocol
FC protocol for ACAT2 antibody 14755-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Biochem Biophys Res Commun

Exosomal and extracellular HMGB1 have opposite effects on SASH1 expression in rat astrocytes and glioma C6 cells.

Authors - Chao Ma
humanWB

Food Res Int

Wild Lonicera caerulea berry polyphenol extract reduces cholesterol accumulation and enhances antioxidant capacity in vitro and in vivo.

Authors - Suwen Liu
mouseWB

J Agric Food Chem

Oral Administration of Succinoglycan Riclin Improves Diet-Induced Hypercholesterolemia in Mice.

Authors - Yunxia Yang
humanWB

Cell Res

Hepatocellular carcinoma redirects to ketolysis for progression under nutrition deprivation stress.

Authors - De Huang

Cell Res

Blocking FSH inhibits hepatic cholesterol biosynthesis and reduces serum cholesterol.

Authors - Yanjing Guo