Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis of HepG2 using 14755-1-AP
WB result of ACAT2 antibody (14755-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACAT2 transfected HepG2 cells.
WB analysis using 14755-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14755-1-AP (ACAT2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
IP experiment of mouse liver using 14755-1-AP
IP result of anti-ACAT2 (IP:14755-1-AP, 4ug; Detection:14755-1-AP 1:1000) with mouse liver tissue lysate 4000ug.
IHC staining of human liver using 14755-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 14755-1-AP (ACAT2 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human liver using 14755-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 14755-1-AP (ACAT2 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human placenta using 14755-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 14755-1-AP (ACAT2 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human placenta using 14755-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 14755-1-AP (ACAT2 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human lung using 14755-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 14755-1-AP (ACAT2 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human lung using 14755-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 14755-1-AP (ACAT2 antibody) at dilution of 1:100 (under 40x lens).
IF Staining of HepG2 using 14755-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ACAT2 antibody (14755-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).
FC experiment of HepG2 using 14755-1-AP
1x10^6 HepG2 cells were intracellularly stained with 0.4 ug ACAT2 Polyclonal antibody (14755-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

Featured Product
KD/KO Validated

ACAT2 Polyclonal antibody

Name: ACAT2 Polyclonal antibody
Brand: Proteintech
SKU: 14755-1-AP
Rating: 5.00 (2 reviews)

ACAT2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 14755-1-AP

Publications(12)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

acetoacetyl CoA thiolase, Acetyl-CoA acetyltransferase, cytosolic, Acetyl-CoA transferase-like protein, ACTL, Cytosolic acetoacetyl-CoA thiolase

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 555 CoraLite® Plus 647

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (12)
Protocols
Reviews (2)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	Caco-2 cells, HepG2 cells, K-562 cells, MOLT-4 cells, mouse liver, rat liver
Positive IP detected in	mouse liver tissue
Positive IHC detected in	human liver tissue, human lung tissue, human placenta tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells
Positive FC (Intra) detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:12000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 8 publications below
IHC	See 3 publications below

Product Information

14755-1-AP targets ACAT2 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag6503 Product name: Recombinant human ACAT2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-397 aa of BC000408 Sequence: MNAGSDPVVIVSAARTIIGSFNGALAAVPVQDLGSTVIKEVLKRATVAPEDVSEVIFGHVLAAGCGQNPVRQASVGAGIPYSVPAWSCQMICGSGLKAVCLAVQSIGIGDSSIVVAGGMENMSKAPHLAYLRTGVKIGEMPLTDSILCDGLTDAFHNCHMGITAENVAKKWQVSREDQDKVAVLSQNRTENAQKAGHFDKEIVPVLVSTRRGLIEVKTDEFPRHGSNIEAMSKLKPYFLTDGTGTVTPANASGINDGAAAVVLMKKSEADKRGLTPLARIVSWSQVGVEPSIMGIGPIPAIKQAVTKAGWSLEDVDIFEINEAFAAVSAAIVKELGLNPEKVNIEGGAIALGHPLGASGCRILVTLLHTLERMGRSRGVAALCIGGGMGIAMCVQRE Predict reactive species
Full Name	acetyl-Coenzyme A acetyltransferase 2
Calculated Molecular Weight	41 kDa
Observed Molecular Weight	40-42 kDa
GenBank Accession Number	BC000408
Gene Symbol	ACAT2
Gene ID (NCBI)	39
RRID	AB_2220236
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9BWD1
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for ACAT2 antibody 14755-1-AP	Download protocol
IHC protocol for ACAT2 antibody 14755-1-AP	Download protocol
IP protocol for ACAT2 antibody 14755-1-AP	Download protocol
WB protocol for ACAT2 antibody 14755-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
		Cell Res Blocking FSH inhibits hepatic cholesterol biosynthesis and reduces serum cholesterol. Authors - Yanjing Guo View Article
human	WB	Cell Res Hepatocellular carcinoma redirects to ketolysis for progression under nutrition deprivation stress. Authors - De Huang View Article
human	IHC	Cancer Cell Int Polyamine metabolism patterns characterized tumor microenvironment, prognosis, and response to immunotherapy in colorectal cancer Authors - Enkui Zhang View Article
mouse	WB	iScience Hepatocyte activity of the cholesterol sensor smoothened regulates cholesterol and bile acid homeostasis in mice. Authors - George D Dalton View Article
mouse	WB	J Agric Food Chem Oral Administration of Succinoglycan Riclin Improves Diet-Induced Hypercholesterolemia in Mice. Authors - Yunxia Yang View Article
human	WB	Food Res Int Wild Lonicera caerulea berry polyphenol extract reduces cholesterol accumulation and enhances antioxidant capacity in vitro and in vivo. Authors - Suwen Liu View Article

Reviews

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The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH Susmita (Verified Customer) (10-29-2025)	works good Applications: Western Blot Primary Antibody Dilution: 1:4000 for WB Cell Tissue Type: Granulosa cell line
FH Sarah-Eve (Verified Customer) (03-01-2023)	Very good working on PVDF membrane, blocking solution milk 5% Applications: Western Blot Primary Antibody Dilution: 1:2000 Cell Tissue Type: Human pulmonary artery smooth muscle cells ACAT2 Antibody Western Blot validation (1:2000 dilution) in Human pulmonary artery smooth muscle cells (Cat no:14755-1-AP)