Tested Applications
| Positive WB detected in | human skeletal muscle tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue |
| Positive IHC detected in | human skeletal muscle tissue, human hysteromyoma tissue, mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | C2C12 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17521-1-AP targets ACTA1-Specific in WB, IHC, IF, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | actin, alpha 1, skeletal muscle |
| Calculated Molecular Weight | 42 kDa |
| Observed Molecular Weight | 39-42 kDa |
| GenBank Accession Number | NM_001100 |
| Gene Symbol | ACTA1 |
| Gene ID (NCBI) | 58 |
| RRID | AB_2273608 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P68133 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. The ACTA1 gene encodes skeletal muscle alpha-actin, the principal actin isoform in adult skeletal muscle, which interacts with a variety of proteins to produce the force for muscle contraction. There is only the N-termninal 8 amino acids difference among the actin family members. This antibody is specific to the ACTA1. It does not cross-react with other actin isoforms.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for ACTA1-Specific antibody 17521-1-AP | Download protocol |
| IHC protocol for ACTA1-Specific antibody 17521-1-AP | Download protocol |
| WB protocol for ACTA1-Specific antibody 17521-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ACTA1-Specific Polyclonal antibody (Cat# 17521-1-AP) has 16 total citations spanning journals including Nature Communications, Circulation Research, Journal of Clinical Investigation, Cancer Letters, iScience, and others. Research fields covered include cardiac hypertrophy and fibrosis, skeletal muscle disorders (myopathy, nemaline myopathy), cancer (colorectal, rhabdomyosarcoma, squamous cell carcinoma), pulmonary arterial hypertension, hepatic fibrosis, myocardial ischemia, and synaptic plasticity in cerebral hypoperfusion models.
| Species | Application | Title |
|---|---|---|
Circ Res Integrated Proteomics Unveils Nuclear PDE3A2 as a Regulator of Cardiac Myocyte Hypertrophy | ||
J Clin Invest Impaired activity of the fusogenic micropeptide Myomixer causes myopathy resembling Carey-Fineman-Ziter syndrome. | ||
Cancer Lett Cancer-associated fibroblasts-derived exosomal miR-17-5p promotes colorectal cancer aggressive phenotype by initiating a RUNX3/MYC/TGF-β1 positive feedback loop. | ||
Aging Dis Galectin-3 Mediates Endothelial-to-Mesenchymal Transition in Pulmonary Arterial Hypertension. | ||
Biomed Pharmacother Novel small molecule DMAMCL induces differentiation in rhabdomyosarcoma by downregulating of DLL1 |
Reviews
What customers say
The single review for 17521-1-AP is highly positive, rated 5 out of 5 stars. The reviewer, A.P., used the antibody for Western Blot (lot 00114409) and reported that it works well and shows good specificity. They also noted that it performs effectively for immunofluorescence on mouse tissues, indicating cross-reactivity beyond its primary application. Overall, the feedback highlights reliable performance and broad utility across applications and species.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
ARUN (Verified Customer) (07-29-2025) | works well and specific. It even works for mouse tissues immunofluorescence.
|
