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beta Actin Antibody 235 Publications

Rabbit Polyclonal| Catalog number: 20536-1-AP

Featured Product KD/KO validated

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Con: 65 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
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Images See all

beta Actin Antibody WB HEK-293 cells 20536-1-AP

Various lysates were subjected to SDS PAGE followed by western blot with 20536-1-AP (beta Actin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours

beta Actin Antibody WB HeLa cells 20536-1-AP

Various lysates were subjected to SDS PAGE followed by western blot with 20536-1-AP (beta Actin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours

beta Actin Antibody WB A549 cells 20536-1-AP

A549 cells (shcontrol and shRNA of Beta actin) were subjected to SDS PAGE followed by western blot with 20536-1-AP(ACTB antibody) at dilution of 1:500.

beta Actin Antibody IF HepG2 cells 20536-1-AP

Immunofluorescent analysis of HepG2 cells, using ACTB antibody 20536-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

beta Actin Antibody IHC human colon tissue 20536-1-AP

Immunohistochemistry of paraffin-embedded human colon tissue slide using 20536-1-AP( beta actin Antibody) at dilution of 1:200 (under 10x lens).

beta Actin Antibody IHC human colon tissue 20536-1-AP

Immunohistochemistry of paraffin-embedded human colon tissue slide using 20536-1-AP( beta actin Antibody) at dilution of 1:200 (under 40x lens).

Species specificity:
human, mouse, rat, zebrafish, monkey, goat, Hamster, pig, Plateau pika
Positive WB detected in:
HEK-293 cells, rat skin tissue, A549 cells, C6 cells, HeLa cells, mouse brain tissue, mouse liver tissue, rat spleen tissue, mouse lung tissue, NIH/3T3 cells, mouse spleen tissue
Positive IHC detected in:
human colon tissue, human heart tissue, human kidney tissue, human skeletal muscle tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HepG2 cells
Recommended dilution:
WB : 1:5000-1:15000
IHC : 1:50-1:500
IF : 1:10-1:100

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
beta Actin fusion protein Ag14521
Full name:
actin, beta
Calculated molecular weight:
375aa,42 kDa
Observed molecular weight:
42 kDa
GenBank accession number:
BC002409
Gene ID (NCBI):
60
Gene symbol
ACTB
Synonyms
ACTB, Actin, actin, beta, Actin, cytoplasmic 1, B actin, Beta actin, F actin, PS1TP5BP1, β actin

Background

Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 37-40 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody was generated against N-terminal region of human beta actin protein and can cross-react with other actins. (9173887, 11217076)


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