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  • Featured Product
  • KD/KO Validated

Beta Actin Polyclonal antibody

Beta Actin Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey, canine and More (8)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 20536-1-AP

Synonyms

ACTB, Actin, actin, beta, Actin, cytoplasmic 1, B actin, Beta actin, Cytoskeleton Marker, F actin, PS1TP5BP1, β actin

Validation Data Gallery

Applications:
  • WB analysis using 20536-1-AP

    WB analysis using 20536-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20536-1-AP (beta Actin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis using 20536-1-AP

    WB analysis using 20536-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20536-1-AP (beta Actin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. beta actin is not expressed in adult heart thus we don't recommend it for heart tissue detection.

  • WB analysis of A549 cells using 20536-1-AP

    WB analysis of A549 cells using 20536-1-AP

    A549 cells (shcontrol and shRNA of Beta actin) were subjected to SDS PAGE followed by western blot with 20536-1-AP (ACTB antibody) at dilution of 1:500.

  • WB analysis using 20536-1-AP

    WB analysis using 20536-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20536-1-AP (beta Actin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 20536-1-AP

    WB analysis using 20536-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20536-1-AP (beta Actin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis using 20536-1-AP

    WB analysis using 20536-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20536-1-AP (beta Actin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • WB analysis of C6 using 20536-1-AP

    WB analysis of C6 using 20536-1-AP

    C6 cells were subjected to SDS PAGE followed by western blot with 20536-1-AP (beta actin Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 20536-1-AP

    IHC staining of human colon using 20536-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20536-1-AP (beta actin Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human colon using 20536-1-AP

    IHC staining of human colon using 20536-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20536-1-AP (beta actin Antibody) at dilution of 1:200 (under 40x lens).

  • IHC staining of human kidney using 20536-1-AP

    IHC staining of human kidney using 20536-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20536-1-AP (beta actin Antibody) at dilution of 1:200 (under 40x lens).

  • IHC staining of human kidney using 20536-1-AP

    IHC staining of human kidney using 20536-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20536-1-AP (beta actin Antibody) at dilution of 1:200 (under 10x lens).

  • IF Staining of MDCK using 20536-1-AP

    IF Staining of MDCK using 20536-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MDCK cells using Beta Actin antibody (20536-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of HepG2 using 20536-1-AP

    FC experiment of HepG2 using 20536-1-AP

    1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human Beta Actin (20536-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

Tested Applications

Positive WB detected inHEK-293 cells, A549 cells, C6 cells, NIH/3T3 cells, mouse colon tissue
Positive IHC detected inhuman colon tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMDCK cells
Positive FC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®650 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 20536-1-AP is Beta Actin Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat, monkey, canine
Cited Reactivityhuman, bovine, Camelus bactrianus, canine, carp, chicken, fish, goat, grouper, Hamster
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Beta Actin fusion protein Ag14521
Full Name actin, beta
Calculated molecular weight 375 aa, 42 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC002409
Gene symbol ACTB
Gene ID (NCBI) 60
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Beta Actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 37-40, 31, 15 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody was generated against N-terminal region of human beta actin protein and can cross-react with other actins. (9173887, 11217076, 10229193 )

Protocols

Product Specific Protocols
WB protocol for Beta Actin antibody 20536-1-APDownload protocol
IHC protocol for Beta Actin antibody 20536-1-APDownload protocol
IF protocol for Beta Actin antibody 20536-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Sci

DEP domain containing 1 predicts prognosis of hepatocellular carcinoma patients and regulates tumor proliferation and metastasis.

Authors - Di Qu
View Article
humanWB

Br J Cancer

Interferon-alpha promotes immunosuppression through IFNAR1/STAT1 signalling in head and neck squamous cell carcinoma.

Authors - Hailong Ma
View Article
humanWB

Theranostics

Adipocytes fuel gastric cancer omental metastasis via PITPNC1-mediated fatty acid metabolic reprogramming.

Authors - Yujing Tan
View Article
humanWB

Nat Commun

Cancer-derived exosomal miR-25-3p promotes pre-metastatic niche formation by inducing vascular permeability and angiogenesis.

Authors - Zhicheng Zeng
View Article
ratWB

Redox Biol

Citrate reduced oxidative damage in stem cells by regulating cellular redox signaling pathways and represent a potential treatment for oxidative stress-induced diseases.

Authors - Xiaopei Wu
View Article
ratWB

Mol Nutr Food Res

Sulforaphane Improves Lipid Metabolism by Enhancing Mitochondrial Function and Biogenesis in vivo and vitro.

Authors - Peng Lei
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Aaron (Verified Customer) (12-08-2020)

nice bands achieved

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: Rat epidermal keratinocytes
FH

Wei (Verified Customer) (01-30-2020)

Strong, clear band for basal heart tissues.Good for internal control for heart diseases.

  • Applications: Western Blot,
  • Cell Tissue Type: Mouse Heart
FH

SCOTT (Verified Customer) (10-11-2019)

Used 1/1000 overnight 4 C. overnightHuman Grey matter cortex post mortem material

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: Human Grey matter cortex post mortem
Beta Actin Antibody Western Blot, validation (1/1000 dilution) in Human Grey matter cortex post mortem (Cat no:20536-1-AP)
FH

Dan (Verified Customer) (08-19-2019)

The antibody works

  • Applications: Western Blot, Immunohistochemistry,
FH

Kishor (Verified Customer) (01-30-2019)

Working well for Western Blotting (1:5000)

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: H69, HUCCT-1 and Rat Liver tissue
Beta Actin Antibody Western Blot, validation (1:5000 dilution) in H69, HUCCT-1 and Rat Liver tissue (Cat no:20536-1-AP)