alpha Actinin Antibody
alpha Actinin Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat
IF, IHC, WB, ELISA
Cat no : 11313-2-AP
actinin, alpha 1, ACTN1, alpha Actinin, Alpha actinin 1, F actin cross linking protein, Non muscle alpha actinin 1
|Positive WB detected in||NIH/3T3 cells, rat heart tissue, mouse spleen tissue|
|Positive IHC detected in||human heart tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||mouse heart tissue, NIH/3T3 cells, C2C12 cells|
|Western Blot (WB)||WB : 1:1000-1:5000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
11313-2-AP targets alpha Actinin in IF, IHC, WB, ELISA applications and shows reactivity with human, mouse, rat samples.
|Reactivity||human, mouse, rat|
|Cited Species||mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||alpha Actinin fusion protein Ag1859|
|Full Name||actinin, alpha 1|
|Calculated molecular weight||103 kDa|
|Observed molecular weight||100-105 kDa|
|GenBank accession number||BC015766|
|Gene ID (NCBI)||87|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20oC. Aliquoting is unnecessary for -20oC storage.|
Alpha actinins are highly conserved actin-binding proteins that form antiparallel homodimers with the actin-binding domains on each end of the molecule, allowing crosslinking of actin molecules. There are four alpha actinin isoforms identified: two ubiquitously expressed cytoskeletal isoforms (ACTN1 and ACTN4) and two striated muscle-specific sarcomeric isoforms (ACTN2 and ACTN3). ACTN1 is also alternatively spliced to form smooth muscle or brain specific isoforms. This antibody recognize endogenous ACTN1, while it may cross-react with ACTN2 and ACTN4.
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Jason Johnson (10-28-2019)
Excellent antibody with consistent results at a 1/100 dilution on formalin fixed tissue.
Steeve Menétrey (01-08-2018)
Tested with another actinin mouse. Gave 1:1 ratio actinin:actinin