alpha Actinin Antibody

alpha Actinin Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, IHC, WB, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 11313-2-AP

Synonyms

actinin, alpha 1, ACTN1, alpha Actinin, Alpha actinin 1, F actin cross linking protein, Non muscle alpha actinin 1



Tested Applications

Positive WB detected inNIH/3T3 cells, rat heart tissue, mouse spleen tissue
Positive IHC detected inhuman heart tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse heart tissue, NIH/3T3 cells, C2C12 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11313-2-AP targets alpha Actinin in IF, IHC, WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Reactivity human, mouse, rat
Cited Species mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen alpha Actinin fusion protein Ag1859
Full Name actinin, alpha 1
Calculated molecular weight 103 kDa
Observed molecular weight 100-105 kDa
GenBank accession number BC015766
Gene symbol ACTN1
Gene ID (NCBI) 87
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Aliquoting is unnecessary for -20oC storage.

Background Information

Alpha actinins are highly conserved actin-binding proteins that form antiparallel homodimers with the actin-binding domains on each end of the molecule, allowing crosslinking of actin molecules. There are four alpha actinin isoforms identified: two ubiquitously expressed cytoskeletal isoforms (ACTN1 and ACTN4) and two striated muscle-specific sarcomeric isoforms (ACTN2 and ACTN3). ACTN1 is also alternatively spliced to form smooth muscle or brain specific isoforms. This antibody recognize endogenous ACTN1, while it may cross-react with ACTN2 and ACTN4.

Protocols

Product Specific Protocols
WB protocol for alpha Actinin antibody 11313-2-APDownload protocol
IHC protocol for alpha Actinin antibody 11313-2-APDownload protocol
IF protocol for alpha Actinin antibody 11313-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Biomed Pharmacother

Proteomic analysis for anti-atherosclerotic effect of tetrahydroxystilbene glucoside in rats.

Authors - Yao Wenjuan W
mouseIHC

Exp Eye Res

Dynamic spatiotemporal expression pattern of limbal stem cell putative biomarkers during mouse development.

Authors - Zhi Hou Guo
mouseWB

Theranostics

miR-17-3p Contributes to Exercise-Induced Cardiac Growth and Protects against Myocardial Ischemia-Reperfusion Injury.

Authors - Jing Shi

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Jason Johnson (10-28-2019)

Excellent antibody with consistent results at a 1/100 dilution on formalin fixed tissue.

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1/100
  • Cell Tissue Type: Mouse myocardium
alpha Actinin Antibody Immunohistochemistry, validation (1/100 dilution) in Mouse myocardium (Cat no:11313-2-AP)
FH

Steeve Menétrey (01-08-2018)

Tested with another actinin mouse. Gave 1:1 ratio actinin:actinin

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1'1000
  • Cell Tissue Type: human umbilical veins
No image submitted with review