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ARP2 Polyclonal antibody

ARP2 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10922-1-AP

Synonyms

Actin like protein 2, Actin related protein 2, ACTR2, ARP2



Tested Applications

Positive WB detected inHeLa cells, mouse kidney tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10922-1-AP targets ARP2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARP2 fusion protein Ag1359
Full Name ARP2 actin-related protein 2 homolog (yeast)
Calculated molecular weight 45 kDa
Observed molecular weight 43-45 kDa
GenBank accession numberBC014546
Gene symbol ACTR2
Gene ID (NCBI) 10097
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ARP2 antibody 10922-1-APDownload protocol
IHC protocol for ARP2 antibody 10922-1-APDownload protocol
IF protocol for ARP2 antibody 10922-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Biol Reprod

RAB8A GTPase regulates spindle migration and Golgi apparatus distribution via ROCK-mediated actin assembly in mouse oocyte meiosis.

Authors - Zhen-Nan Pan
humanIF

Elife

mTOR signaling regulates the morphology and migration of outer radial glia in developing human cortex.

Authors - Madeline G Andrews
humanWB

Nat Cell Biol

miR-129-3p controls cilia assembly by regulating CP110 and actin dynamics.

Authors - Cao Jingli J
  • KD Validated