ADAM10 Monoclonal antibody

ADAM10 Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

2A6B7

Cat no : 66620-1-Ig

Synonyms

AD10, ADAM 10, ADAM10, CD156c, CDw156, HsT18717, kuz, Kuzbanian protein homolog, MADM



Tested Applications

Positive WB detected inTHP-1 cells, MCF-7 cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman lung cancer tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Sample-dependent, check data in validation data gallery

Product Information

66620-1-Ig targets ADAM10 in WB, IHC,ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ADAM10 fusion protein Ag23062
Full Name ADAM metallopeptidase domain 10
Calculated molecular weight 748 aa, 84 kDa
Observed molecular weight 90-95 kDa
GenBank accession numberBC126253
Gene symbol ADAM10
Gene ID (NCBI) 102
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ADAM10, a member of the ADAM (a disintegrin and metalloprotease) family, is widely expressed in the brain, the spinal cord, and the visual system during development.MW of premature ADAM10 is 90 kDa and mature ADAM10 is 60-70 kDa (PMID: 24404179).

Protocols

Product Specific Protocols
WB protocol for ADAM10 antibody 66620-1-IgDownload protocol
IHC protocol for ADAM10 antibody 66620-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols