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  • KD/KO Validated

Gamma Adducin Polyclonal antibody

Gamma Adducin Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 17585-1-AP

Synonyms

ADD3, ADDL, adducin 3 (gamma), Adducin like protein 70, Gamma adducin



Tested Applications

Positive WB detected inNIH/3T3 cells, K-562 cells, mouse kidney tissue
Positive IP detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17585-1-AP targets Gamma Adducin in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Gamma Adducin fusion protein Ag11318
Full Name adducin 3 (gamma)
Calculated Molecular Weight 76 kDa, 79 kDa
Observed Molecular Weight 80 kDa, 90 kDa
GenBank Accession NumberBC062559
Gene Symbol ADD3
Gene ID (NCBI) 120
RRIDAB_2223579
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for Gamma Adducin antibody 17585-1-APDownload protocol
IP protocol for Gamma Adducin antibody 17585-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Mol Cell Biol

QKI-5 regulates the alternative splicing of cytoskeletal gene ADD3 in lung cancer.

Authors - Jin-Zhu Wang
humanWB

Stem Cell Res Ther

Knockdown of long noncoding RNA SAN rejuvenates aged adipose-derived stem cells via miR-143-3p/ADD3 axis

Authors - Hewei Xiong
  • KD Validated
humanWB

PLoS One

Dysferlin and other non-red cell proteins accumulate in the red cell membrane of diamond-blackfan anemia patients.

Authors - Esther N Pesciotta
humanWB

Cell Genom

Protein interaction studies in human induced neurons indicate convergent biology underlying autism spectrum disorders

Authors - Greta Pintacuda