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ADH4 Monoclonal antibody

ADH4 Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

3D12E9

Cat no : 67303-1-Ig

Synonyms

ADH 2, ADH4, Alcohol dehydrogenase 4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ADH4 Monoclonal antibody (67303-1-Ig)

    WB analysis using 67303-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67303-1-Ig (ADH4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using ADH4 Monoclonal antibody (67303-1-Ig)

    IF Staining of HEK-293 using 67303-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using ADH4 antibody (67303-1-Ig, Clone: 3D12E9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inHepG2 cells, L02 cells, HuH-7 cells
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67303-1-Ig targets ADH4 in WB, IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ADH4 fusion protein Ag9884
Full Name alcohol dehydrogenase 4 (class II), pi polypeptide
Calculated Molecular Weight 380 aa, 40 kDa
Observed Molecular Weight 40-43 kDa
GenBank Accession NumberBC022319
Gene Symbol ADH4
Gene ID (NCBI) 127
RRIDAB_2882565
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ADH4 antibody 67303-1-IgDownload protocol
IF protocol for ADH4 antibody 67303-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols