CoraLite® Plus 488-conjugated ADH4 Monoclonal antibody

ADH4 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3D12E9

Cat no : CL488-67303

Synonyms

ADH 2, ADH4, Alcohol dehydrogenase 4



Tested Applications

Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

CL488-67303 targets ADH4 in IF applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ADH4 fusion protein Ag9884
Full Name alcohol dehydrogenase 4 (class II), pi polypeptide
Calculated molecular weight 380 aa, 40 kDa
Observed molecular weight 40-43 kDa
GenBank accession numberBC022319
Gene symbol ADH4
Gene ID (NCBI) 127
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

This gene encodes the class II alcohol dehydrogenase 4 pi subunit, which is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class II alcohol dehydrogenase is a homodimer composed of 2 pi subunits. ADH4 plays a critical role in alcoholism development and alcohol toxicology and is also associated with the development of esophageal cancer(PMID:33556337, 26039424, 24051444, 22147505).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 ADH4 antibody CL488-67303Download protocol
Standard Protocols
Click here to view our Standard Protocols