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Tested Applications
| Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
| IF | See 2 publications below |
Product Information
19778-1-AP targets ADRA2B-Specific in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide Predict reactive species |
| Full Name | adrenergic, alpha-2B-, receptor |
| Calculated Molecular Weight | 50 kDa |
| GenBank Accession Number | NM_000682 |
| Gene Symbol | ADRA2B |
| Gene ID (NCBI) | 151 |
| RRID | AB_10640910 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P18089 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ADRA2B, also named as ADRA2L1 and ADRA2RL1, belongs to the G-protein coupled receptor 1 family. ADRA2B mediates the catecholamine-induced inhibition of adenylate cyclase through the action of G proteins. The antibody is specific to ADRA2B.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ADRA2B-Specific antibody 19778-1-AP | Download protocol |
| FC protocol for ADRA2B-Specific antibody 19778-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Tissue Res Subcellular distribution of α2-adrenoceptor subtypes in the rodent kidney. | ||
Eur J Pharmacol Yohimbine ameliorates lipopolysaccharide-induced acute kidney injury in rats. | ||
J Pharmacol Sci Inhibition of α2-adrenoceptor is renoprotective in 5/6 nephrectomy-induced chronic kidney injury rats. |
