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ADRA2B-Specific Polyclonal antibody

ADRA2B-Specific Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC, ELISA

Conjugate

Unconjugated

Cat no : 19778-1-AP

Synonyms

ADRA2B, ADRA2L1, ADRA2RL1, ADRARL1, Alpha 2B adrenergic receptor, Alpha 2B adrenoceptor, Alpha 2B adrenoreceptor, Alpha 2BAR, ALPHA2BAR

Validation Data Gallery

  • WB analysis of mouse brain using 19778-1-AP

    WB analysis of mouse brain using 19778-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 19778-1-AP (ADRA2B-Specific antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 19778-1-AP

    IHC staining of mouse brain using 19778-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19778-1-AP (ADRA2B-Specific Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 19778-1-AP

    IHC staining of mouse brain using 19778-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19778-1-AP (ADRA2B-Specific Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of HEK-293 using 19778-1-AP

    FC experiment of HEK-293 using 19778-1-AP

    1X10^6 HEK-293 cells were stained with 0.2ug ADRA2B-Specific antibody (19778-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

Tested Applications

Positive WB detected inmouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

19778-1-AP targets ADRA2B-Specific in WB, IHC, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name adrenergic, alpha-2B-, receptor
Calculated molecular weight 50 kDa
Observed molecular weight 48-50 kDa
GenBank accession numberNM_000682
Gene symbol ADRA2B
Gene ID (NCBI) 151
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ADRA2B, also named as ADRA2L1 and ADRA2RL1, belongs to the G-protein coupled receptor 1 family. ADRA2B mediate the catecholamine-induced inhibition of adenylate cyclase through the action of G proteins. The antibody is specific to ADRA2B.

Protocols

Product Specific Protocols
WB protocol for ADRA2B-Specific antibody 19778-1-APDownload protocol
IHC protocol for ADRA2B-Specific antibody 19778-1-APDownload protocol
FC protocol for ADRA2B-Specific antibody 19778-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Eur J Pharmacol

Yohimbine ameliorates lipopolysaccharide-induced acute kidney injury in rats.

Authors - Takaomi Shimokawa
View Article