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ADRA2B-Specific Antibody 0 Publications

Rabbit Polyclonal| Catalog number: 19778-1-AP

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Con: 21 μg/150 μl

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Tested Applications

Applications:
WB, IHC, FC, ELISA
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Images See all

ADRA2B-Specific Antibody WB mouse brain tissue 19778-1-AP

mouse brain tissue were subjected to SDS PAGE followed by western blot with 19778-1-AP(ADRA2B-Specific antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours

ADRA2B-Specific Antibody IHC mouse brain tissue 19778-1-AP

Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 19778-1-AP( ADRA2B-Specific Antibody) at dilution of 1:100 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

ADRA2B-Specific Antibody IHC mouse brain tissue 19778-1-AP

Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 19778-1-AP( ADRA2B-Specific Antibody) at dilution of 1:100 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

ADRA2B-Specific Antibody FC HEK-293 cells 19778-1-AP

1X10^6 HEK-293 cells were stained with 0.2ug ADRA2B-Specific antibody (19778-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

Species specificity:
human, mouse, rat
Positive WB detected in:
mouse brain tissue
Positive IHC detected in:
mouse brain tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected in:
HEK-293 cells
Recommended dilution:
WB : 1:200-1:1000
IHC : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Peptide
Full name:
adrenergic, alpha-2B-, receptor
Calculated molecular weight:
50 kDa
Observed molecular weight:
48-50 kDa
GenBank accession number:
NM_000682
Gene ID (NCBI):
151
Gene symbol
ADRA2B
Synonyms
ADRA2B, ADRA2L1, ADRA2RL1, ADRARL1, Alpha 2B adrenergic receptor, Alpha 2B adrenoceptor, Alpha 2B adrenoreceptor, Alpha 2BAR, ALPHA2BAR

Background

ADRA2B, also named as ADRA2L1 and ADRA2RL1, belongs to the G-protein coupled receptor 1 family. ADRA2B mediate the catecholamine-induced inhibition of adenylate cyclase through the action of G proteins. The antibody is specific to ADRA2B.


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