|Positive WB detected in||human brain tissue, BxPC-3 cells, HepG2 cells, human heart tissue, rat brain tissue, SH-SY5Y cells, SW 1990 cells|
|Positive IP detected in||HepG2 cells|
|Positive IHC detected in||human liver cancer tissue, human ovary tumor tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
14550-1-AP targets AFP in WB, Pull-down, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||AFP fusion protein Ag6089|
|Calculated molecular weight||69 kDa|
|Observed molecular weight||68-72 kDa|
|GenBank accession number||BC027881|
|Gene ID (NCBI)||174|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
AFP, also known as Alpha-fetoprotein, is a major plasma protein in the fetus and its concentration is very low in the adult. So AFP is usually considered a marker of hepatocyte precursors. AFP can be detected at abnormally high concentrations in hepatocellular carcinomas as well as in the plasma and ascitic fluid of adults with hepatoma. High AFP concentrations have been correlated with tumor cell growth, indicating that AFP can serve as a tumor marker.
|Product Specific Protocols|
|WB protocol for AFP antibody 14550-1-AP||Download protocol|
|IHC protocol for AFP antibody 14550-1-AP||Download protocol|
|IF protocol for AFP antibody 14550-1-AP||Download protocol|
|IP protocol for AFP antibody 14550-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Breakdown of adaptive immunotolerance induces hepatocellular carcinoma in HBsAg-tg mice.
RACK1 Promotes Self-Renewal and Chemoresistance of Cancer Stem Cells in Human Hepatocellular Carcinoma through Stabilizing Nanog.
Am J Pathol
TEA Domain Transcription Factor 4 Is the Major Mediator of Yes-Associated Protein Oncogenic Activity in Mouse and Human Hepatoblastoma.
Hepatobiliary Pancreat Dis Int
Au@SiO2@CuInS2-ZnS/Anti-AFP fluorescent probe improves HCC cell labeling.
World J Gastroenterol
Signal transducer and activator of transcription 3 promotes the Warburg effect possibly by inducing pyruvate kinase M2 phosphorylation in liver precancerous lesions.
Cancer Manag Res
Peri-tumor fibroblasts promote tumorigenesis and metastasis of hepatocellular carcinoma via Interleukin6/STAT3 signaling pathway.