AFP Polyclonal antibody

AFP Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Pull-down, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 14550-1-AP

Synonyms

AFP, Alpha 1 fetoprotein, Alpha fetoglobulin, alpha fetoprotein, FETA, HPAFP



Tested Applications

Positive WB detected inhuman brain tissue, BxPC-3 cells, HepG2 cells, human heart tissue, rat brain tissue, SH-SY5Y cells, SW 1990 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

14550-1-AP targets AFP in WB, Pull-down, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AFP fusion protein Ag6089
Full Name alpha-fetoprotein
Calculated molecular weight 69 kDa
Observed molecular weight 68-72 kDa
GenBank accession numberBC027881
Gene symbol AFP
Gene ID (NCBI) 174
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AFP, also known as Alpha-fetoprotein, is a major plasma protein in the fetus and its concentration is very low in the adult. So AFP is usually considered a marker of hepatocyte precursors. AFP can be detected at abnormally high concentrations in hepatocellular carcinomas as well as in the plasma and ascitic fluid of adults with hepatoma. High AFP concentrations have been correlated with tumor cell growth, indicating that AFP can serve as a tumor marker.

Protocols

Product Specific Protocols
WB protocol for AFP antibody 14550-1-APDownload protocol
IHC protocol for AFP antibody 14550-1-APDownload protocol
IF protocol for AFP antibody 14550-1-APDownload protocol
IP protocol for AFP antibody 14550-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IHC

Nat Commun

Breakdown of adaptive immunotolerance induces hepatocellular carcinoma in HBsAg-tg mice.

Authors - Lu Zong
humanIHC

Theranostics

RACK1 Promotes Self-Renewal and Chemoresistance of Cancer Stem Cells in Human Hepatocellular Carcinoma through Stabilizing Nanog.

Authors - Junxia Cao
humanWB

Am J Pathol

TEA Domain Transcription Factor 4 Is the Major Mediator of Yes-Associated Protein Oncogenic Activity in Mouse and Human Hepatoblastoma.

Authors - Jie Zhang
human

Hepatobiliary Pancreat Dis Int

Au@SiO2@CuInS2-ZnS/Anti-AFP fluorescent probe improves HCC cell labeling.

Authors - Yi-Wen Dai
ratWB

World J Gastroenterol

Signal transducer and activator of transcription 3 promotes the Warburg effect possibly by inducing pyruvate kinase M2 phosphorylation in liver precancerous lesions.

Authors - Yang-Hui Bi
humanIHC

Cancer Manag Res

Peri-tumor fibroblasts promote tumorigenesis and metastasis of hepatocellular carcinoma via Interleukin6/STAT3 signaling pathway.

Authors - Zhenxiong Zhao