CoraLite®488-conjugated AKT Monoclonal antibody

AKT Monoclonal Antibody for IF, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF, ELISA

Conjugate

CoraLite®488 Fluorescent Dye

CloneNo.

2C5D1

Cat no : CL488-60203

Synonyms

AKT, AKT1, PKB, PKB ALPHA, PRKBA, Protein kinase B, Proto oncogene c Akt, RAC, RAC ALPHA, RAC PK alpha



Tested Applications

Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:100
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of CL488-60203 is AKT Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen AKT fusion protein Ag16695
Full Name v-akt murine thymoma viral oncogene homolog 1
Calculated molecular weight 56 kDa
GenBank accession numberBC000479
Gene symbol AKT1
Gene ID (NCBI) 207
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission maxima wavelengths488 nm/515 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. This antibody detects all the members of AKT with/without phospho- modification. This antibody is CL488(Ex/Em 488 nm/515 nm) conjugated.

Protocols

Product Specific Protocols
IF protocol for CL488-conjugated AKT antibody CL488-60203Download protocol
Standard Protocols
Click here to view our Standard Protocols