Tested Applications
| Positive WB detected in | L02 cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
82915-2-RR targets ALPL in WB, FC (Intra), ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag1664 Product name: Recombinant human ALPL protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 23-330 aa of BC021289 Sequence: EKDPKYWRDQAQETLKYALELQKLNTNVAKNVIMFLGDGMGVSTVTAARILKGQLHHNPGEETRLEMDKFPFVALSKTYNTNAQVPDSAGTATAYLCGVKANEGTVGVSAATERSRCNTTQGNEVTSILRWAKDAGKSVGIVTTTRVNHATPSAAYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNIRDIDVIMGGGRKYMYPKNKTDVEYESDEKARGTRLDGLDLVDTWKSFKPRYKHSHFIWNRTELLTLDPHNVDYLLGLFEPGDMQYELNRNNVTDPSLSEMVVVAIQILRKNPKGFFLL Predict reactive species |
| Full Name | alkaline phosphatase, liver/bone/kidney |
| Calculated Molecular Weight | 57 kDa |
| Observed Molecular Weight | 80 kDa |
| GenBank Accession Number | BC021289 |
| Gene Symbol | ALPL |
| Gene ID (NCBI) | 249 |
| RRID | AB_3670648 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P05186 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The product of this gene is a membrane bound glycosylated enzyme that is expressed in multiple tissues including liver, bone, and kidney, rather than being restricted to a single tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. ALPL(Alkaline phosphatase, tissue-nonspecific isozyme) is also named as AP-TNAP, TNSALP and belongs to the alkaline phosphatase family. It can exsit as a homodimer with the molecular weight of 140 kDa(PMID:18724009). ALPL was synthesized as a 66-kDa endo-b-N-acetylglucos-aminidase H (Endo H)-sensitive form, and processed to an 80-kDa mature form, which is anchored to the plasma membrane via glycosylphosphatidylinositol (GPI)(PMID:10839996).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for ALPL antibody 82915-2-RR | Download protocol |
| WB protocol for ALPL antibody 82915-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ALPL Recombinant monoclonal antibody (230212D5), catalog number 82915-2-RR, has 2 total citations. These appear in Biochemical Pharmacology and bioRxiv. The cited research covers traumatic heterotopic ossification involving TGF-β2-driven endothelial-mesenchymal transition, and the FNDC1-NAMPT-NAD axis implicated in arterial disease and vascular calcification. The antibody was applied in Western blot (WB) for human samples.
| Species | Application | Title |
|---|---|---|
Biochem Pharmacol SIS3 attenuates traumatic heterotopic ossification by targeting TGF-β2-driven endothelial-mesenchymal transition. | ||
bioRxiv A Novel FNDC1-NAMPT-NAD axis is Implicated in Small and Large-vessel Arterial Disease and Drives Vascular Calcification. |
