Tested Applications
| Positive WB detected in | human placenta tissue |
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human placenta tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
84813-1-RR targets ALPPL2 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag13360 Product name: Recombinant human ALPPL2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 223-532 aa of BC014139 Sequence: RKYMFPMGTPDPEYPDDYSQGGTRLDGKNLVQEWLAKRQGARYVWNRTELMQASLDPSVTHLMGLFEPGDMKYEIHRDSTLDPSLMEMTEAALRLLSRNPRGFFLFVEGGRIDHGHHESRAYRALTETIMFDDAIERAGQLTSEEDTLSLVTADHSHVFSFGGYPLRGSSIFGLAPGKARDRKAYTVLLYGNGPGYVLKDGARPDVTESESGSPEYRQQSAVPLDGETHAGEDVAVFARGPQAHLVHGVQEQTFIAHVMAFAACLEPYTACDLAPRAGTTDAAHPGPSVVPALLPLLAGTLLLLGTATAP Predict reactive species |
| Full Name | alkaline phosphatase, placental-like 2 |
| Calculated Molecular Weight | 57 kDa |
| Observed Molecular Weight | 70 kDa |
| GenBank Accession Number | BC014139 |
| Gene Symbol | ALPPL2 |
| Gene ID (NCBI) | 251 |
| RRID | AB_3672137 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | P10696 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ALPPL2 (alkaline phosphatase placental-like 2), a member of the human alkaline phosphatase family, could be a potential biomarker for early diagnosis of Pancreatic ductal adenocarcinoma.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ALPPL2 antibody 84813-1-RR | Download protocol |
| IHC protocol for ALPPL2 antibody 84813-1-RR | Download protocol |
| WB protocol for ALPPL2 antibody 84813-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
