ALY Polyclonal antibody

ALY Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16690-1-AP

Synonyms

Ally of AML 1 and LEF 1, ALY, ALYREF, BEF, bZIP enhancing factor BEF, THO complex 4, THO complex subunit 4, Tho4, THOC4



Tested Applications

Positive WB detected inHeLa cells, A431 cells, HEK-293 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 16690-1-AP is ALY Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ALY fusion protein Ag10044
Full Name THO complex 4
Calculated molecular weight 257 aa, 27 kDa
Observed molecular weight 27-30 kDa
GenBank accession numberBC052302
Gene symbol THOC4
Gene ID (NCBI) 10189
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ALY antibody 16690-1-APDownload protocol
IHC protocol for ALY antibody 16690-1-APDownload protocol
IF protocol for ALY antibody 16690-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols