Gamma adaptin Antibody

Gamma adaptin Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat no : 13258-1-AP

Synonyms

ADTG, AP 1 complex subunit gamma 1, AP1G1, CLAPG1, Gamma1 adaptin



Tested Applications

Positive WB detected inHEK-293 cells, mouse skeletal muscle tissue
Positive IHC detected inhuman pancreas cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

13258-1-AP targets Gamma adaptin in IHC, WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Reactivity human, mouse, rat
Cited Species human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Gamma adaptin fusion protein Ag3696
Full Name adaptor-related protein complex 1, gamma 1 subunit
Calculated molecular weight 825aa,92 kDa
Observed molecular weight 91 kDa
GenBank accession number BC036283
Gene symbol AP1G1
Gene ID (NCBI) 164
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Gamma adaptin antibody 13258-1-APDownload protocol
IHC protocol for Gamma adaptin antibody 13258-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Front Cell Dev Biol

Visualization of Protein Sorting at the Trans-Golgi Network and Endosomes Through Super-Resolution Imaging.

Authors - Yan Huang
mouseWB

Front Cell Neurosci

MEA6 Deficiency Impairs Cerebellar Development and Motor Performance by Tethering Protein Trafficking.

Authors - Xin-Tai Wang
humanWB

Nat Cell Biol

Molecular identification of a BAR domain-containing coat complex for endosomal recycling of transmembrane proteins.

Authors - Boris Simonetti