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AP2B1 Polyclonal antibody

AP2B1 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 15690-1-AP

Synonyms

ADTB2, AP 2 complex subunit beta, AP105B, AP2 BETA, AP2B1, Beta 2 adaptin, Beta adaptin, CLAPB1



Tested Applications

Positive WB detected inSH-SY5Y cells, C6 cells, K-562 cells
Positive IP detected inK-562 cells
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

15690-1-AP targets AP2B1 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AP2B1 fusion protein Ag8248
Full Name adaptor-related protein complex 2, beta 1 subunit
Calculated molecular weight 105 kDa
Observed molecular weight 100 kDa-115 kDa
GenBank accession numberBC006201
Gene symbol AP2B1
Gene ID (NCBI) 163
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AP2B1 is a component of the adaptor protein complex 2 (AP-2). AP complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP complexes are involved in the formation of clathrin-coated vesicles (CCVs) by recruiting the scaffold protein, clathrin. AP complexes also play a pivotal role in the cargo selection by recognizing the sorting signals within the cytoplasmic tail of integral membrane proteins. AP-2 works on the plasma membrane to internalize cargo in clathrin-mediated endocytosis.

Protocols

Product Specific Protocols
WB protocol for AP2B1 antibody 15690-1-APDownload protocol
IF protocol for AP2B1 antibody 15690-1-APDownload protocol
IP protocol for AP2B1 antibody 15690-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Exp Clin Cancer Res

GPAA1 promotes gastric cancer progression via upregulation of GPI-anchored protein and enhancement of ERBB signalling pathway.

Authors - Xiao-Xin Zhang
humanIF

Biochem Biophys Res Commun

Trans-Golgi protein p230/golgin-245 is involved in phagophore formation.

Authors - Miwa Sohda
humanWB

J Neuropathol Exp Neurol

Alzheimer Disease Pathology-Associated Polymorphism in a Complex Variable Number of Tandem Repeat Region Within the MUC6 Gene, Near the AP2A2 Gene.

Authors - Yuriko Katsumata
humanWB

J Virol

The G protein-coupled receptor FFAR2 promotes internalization during influenza A virus entry.

Authors - Guangwen Wang
  • KD Validated