AP50 Polyclonal antibody

AP50 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27355-1-AP

Synonyms

Adaptin mu2, AP 2 complex subunit mu, AP 2 mu chain, AP2M1, AP50, CLAPM1, HA2 50 kDa subunit, KIAA0109, mu2



Tested Applications

Positive WB detected inmouse brain tissue, HepG2 cells, rat brain tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 27355-1-AP is AP50 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AP50 fusion protein Ag26059
Full Name adaptor-related protein complex 2, mu 1 subunit
Calculated molecular weight 433 aa, 49 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC013796
Gene symbol AP2M1
Gene ID (NCBI) 1173
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for AP50 antibody 27355-1-APDownload protocol
IHC protocol for AP50 antibody 27355-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols