APAF1 Antibody

APAF1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (1)

Applications

IF, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat no : 21710-1-AP

Synonyms

APAF 1, APAF1, CED4, KIAA0413



Tested Applications

Positive WB detected inHEK-293 cells, A549 cells, COLO 320 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman heart tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 20 publications below

Product Information

The 21710-1-AP immunogen is Fusion Protein APAF1 protein expressed in E. coli.

Reactivity human, rat
Cited Species human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen APAF1 fusion protein Ag16336
Full Name apoptotic peptidase activating factor 1
Calculated molecular weight 1248aa,142 kDa
Observed molecular weight 130-142 kDa
GenBank accession number BC136532
Gene symbol APAF1
Gene ID (NCBI) 317
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Aliquoting is unnecessary for -20oC storage.

Background Information

APAF1, also named as Apoptotic protease-activating factor 1 or KIAA0413, is a 1248 amino acid protein, which contains one CARD domain, contains one NB-ARC domain and Contains fifteen WD repeats. APAF1 localizes in the cytoplasm and is widely expressed in many tissues. Highest levels of expression is in adult spleen, peripheral blood leukocytes, in fetal brain, kidney and lung. Oligomeric APAF1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis.

Protocols

Product Specific Protocols
WB protocol for APAF1 antibody 21710-1-APDownload protocol
IHC protocol for APAF1 antibody 21710-1-APDownload protocol
IF protocol for APAF1 antibody 21710-1-APDownload protocol
IP protocol for APAF1 antibody 21710-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Front Pharmacol

Propofol Ameliorates H9c2 Cells Apoptosis Induced by Oxygen Glucose Deprivation and Reperfusion Injury via Inhibiting High Levels of Mitochondrial Fusion and Fission.

Authors - Lidong Zhao
humanWB

Oxid Med Cell Longev

Apoptotic Protease Activating Factor-1 Inhibitor Mitigates Myocardial Ischemia Injury via Disturbing Procaspase-9 Recruitment by Apaf-1.

Authors - Ying Wang
humanWB

Chin J Traumatol

The subsequent biological effects of simulated microgravity on endothelial cell growth in HUVECs.

Authors - Dan Xu
humanWB

J Agric Food Chem

β-Sitosterol-D-glucoside from sweet potato exerts an anti-breast cancer activity by upregulating microRNA-10a and PI3K/Akt signaling pathway.

Authors - Heshan Xu
humanWB

Oncol Lett

Phloretin induces apoptosis of human esophageal cancer via a mitochondria-dependent pathway.

Authors - Hongtao Duan
humanWB

Biochem Cell Biol

MiR-21 regulates the apoptosis of keloid fibroblasts by caspase-8 and mitochondrial-mediated apoptotic signaling pathway via targeting FasL.

Authors - Ying Liu