Tested Applications
| Positive WB detected in | COLO 320 cells, HeLa cells, human plasma tissue |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
14758-1-AP targets AARE in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6511 Product name: Recombinant human APEH protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 381-732 aa of BC000362 Sequence: AQRSRQDLFAVDTQVGTVTSLTAGGSGGSWKLLTIDQDLMVAQFSTPSLPPTLKVGFLPSAGKEQSVLWVSLEEAEPIPDIHWGIRVLQPPPEQENVQYAGLDFEAILLQPGSPPDKTQVPMVVMPHGGPHSSFVTAWMLFPAMLCKMGFAVLLVNYRGSTGFGQDSILSLPGNVGHQDVKDVQFAVEQVLQEEHFDASHVALMGGSHGGFISCHLIGQYPETYRACVARNPVINIASMLGSTDIPDWCVVEAGFPFSSDCLPDLSVWAEMLDKSPIRYIPQVKTPLLLMLGQEDRRVPFKQGMEYYRALKTRNVPVRLLLYPKSTHALSEVEVESDSFMNAVLWLRTHLGS Predict reactive species |
| Full Name | N-acylaminoacyl-peptide hydrolase |
| Calculated Molecular Weight | 81 kDa |
| Observed Molecular Weight | 66-80 kDa |
| GenBank Accession Number | BC000362 |
| Gene Symbol | APEH |
| Gene ID (NCBI) | 327 |
| RRID | AB_2057811 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P13798 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
APH, (APEH, acylpeptide hydrolase), which catalyzes the hydrolysis of the terminal acetylated amino acid preferentially from small acetylated peptides. The acylpeptide hydrolase is a homotetrameric protein of 300 kDa with each subunit consisting of 732 amino acid residues. A range of molecular masses have been reported for APH by previous investigators, including 80 kD, 75 kD and 69 kD. This antibody can recognize at least 2 isoforms of APH.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for AARE antibody 14758-1-AP | Download protocol |
| IHC protocol for AARE antibody 14758-1-AP | Download protocol |
| WB protocol for AARE antibody 14758-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
