Apolipoprotein AI Polyclonal antibody

Apolipoprotein AI Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 14427-1-AP

Synonyms

Apo AI, ApoA I, APOA1, apolipoprotein A I, Apolipoprotein A1



Tested Applications

Positive WB detected inhuman blood tissue, human brain tissue, human ileum tissue, mouse lung tissue, PC-13 cells
Positive IP detected inmouse lung tissue
Positive IHC detected inhuman liver cancer tissue, human liver tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

14427-1-AP targets Apolipoprotein AI in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Apolipoprotein AI fusion protein Ag5793
Full Name apolipoprotein A-I
Calculated molecular weight 31 kDa
Observed molecular weight 26-30 kDa
GenBank accession numberBC005380
Gene symbol APOA1
Gene ID (NCBI) 335
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ApoA1 is a major protein component of high density lipoproteins (HDL) which is associated with reversed cholesterol transport, lipid/cholesterol binding, lecithin/cholesterol acyltransferase (LCAT) activation and specific receptors binding. It is synthesized in the liver and small intestine. Defects of ApoA1 cause low HDL level and systemic non-neuropathic amyloidosis. Serum concentration of ApoA1 is inversely related to the risk of developing atherosclerosis. This antibody was generated against the C-terminal region of human ApoA1.

Protocols

Product Specific Protocols
WB protocol for Apolipoprotein AI antibody 14427-1-APDownload protocol
IHC protocol for Apolipoprotein AI antibody 14427-1-APDownload protocol
IF protocol for Apolipoprotein AI antibody 14427-1-APDownload protocol
IP protocol for Apolipoprotein AI antibody 14427-1-APDownload protocol
FC protocol for Apolipoprotein AI antibody 14427-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Exp Cell Res

Proteomic analysis of mouse ovaries during the prepubertal stages.

Authors - Jiaqiang Xiong
humanIHC

Br J Cancer

A new panel of pancreatic cancer biomarkers discovered using a mass spectrometry-based pipeline.

Authors - Xiaohui Liu
humanWB

Aging (Albany NY)

Serum proteomic predicts effectiveness and reveals potential biomarkers for complications in liver transplant patients.

Authors - Wenjing Wang
mouseWB

Exp Ther Med

Effects of sepsis on the metabolism of sphingomyelin and cholesterol in mice with liver dysfunction.

Authors - Jiaqi Li
humanWB,IF

Biochimie

Apolipoprotein A-I improves hepatic autophagy through the AMPK pathway.

Authors - Xia Rao
humanIHC

PLoS One

Proteomics Approaches for Identification of Tumor Relevant Protein Targets in Pulmonary Squamous Cell Carcinoma by 2D-DIGE-MS.

Authors - Hao Lihong

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Alexandre (Verified Customer) (02-28-2019)

Detection of both human and mouse APOA1 protein by westernblot Immunoprecipitation of both human and mouse APOA1

  • Applications: Western Blot,Immunoprecipitation,
  • Primary Antibody Dilution: WB 1/1000
  • Cell Tissue Type: HepG2 / Mouse Liver
Apolipoprotein AI Antibody Western Blot,Immunoprecipitation, validation (WB 1/1000 dilution) in HepG2 / Mouse Liver (Cat no:14427-1-AP)