Tested Applications
| Positive WB detected in | HEK-293 cells, human brain tissue, HeLa cells, Jurkat cells, MCF-7 cells |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14282-1-AP targets ARHGDIG in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5554 Product name: Recombinant human ARHGDIG protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-225 aa of BC047699 Sequence: MLGLDACELGAQLLELLRLALCARVLLADKEGGPPAVDEVLDEAVPEYRAPGRKSLLEIRQLDPDDRSLAKYKRVLLGPLPPAVDPSLPNVQVTRLTLLSEQAPGPVVMDLTGDLAVLKDQVFVLKEGVDYRVKISFKVHREIVSGLKCLHHTYRRGLRVDKTVYMVGSYGPSAQEYEFVTPVEEAPRGALVRGPYLVVSLFTDDDRTHHLSWEWGLCICQDWKD Predict reactive species |
| Full Name | Rho GDP dissociation inhibitor (GDI) gamma |
| Calculated Molecular Weight | 25 kDa |
| Observed Molecular Weight | 25 kDa |
| GenBank Accession Number | BC047699 |
| Gene Symbol | ARHGDIG |
| Gene ID (NCBI) | 398 |
| RRID | AB_2059733 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q99819 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ARHGDIG antibody 14282-1-AP | Download protocol |
| WB protocol for ARHGDIG antibody 14282-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ARHGDIG Polyclonal antibody (Cat# 14282-1-AP) has 3 total citations. These appear in Cancer Cell, Chemical Biology & Interactions, and Oncology Reports. The associated research fields include lung cancer dormancy and neutrophil extracellular traps, vascular smooth muscle cell phenotypic switching and intimal hyperplasia via the NLRP3 inflammasome axis, and cervical cancer migration/invasion linked to lncRNA regulation.
| Species | Application | Title |
|---|---|---|
Cancer Cell Chemotherapy awakens dormant cancer cells in lung by inducing neutrophil extracellular traps | ||
Chem Biol Interact The novel piperlongumine-ligustrazine derivative PL-Lig-11 inhibits VSMC phenotypic switching and intimal hyperplasia via the PDGFRα/β-RhoGDI3-NLRP3 inflammasome axis. | ||
Oncol Rep Upregulation and hypomethylation of lncRNA AFAP1‑AS1 predicts a poor prognosis and promotes the migration and invasion of cervical cancer. |
