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ARID1B Recombinant monoclonal antibody (82979-6-RR)

ARID1B Recombinant monoclonal antibody

ARID1B Uni-rAb^® Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA

Cat No. 82979-6-RR

Clone No. 230222A11

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF/ICC, FC (Intra), ELISA

Synonyms

230222A11, BAF250b, BRIGHT, DAN15, ELD/OSA1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

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Filter: AllIHCIF/ICCFC (Intra)Affinity

IHC staining of human placenta using 82979-6-RR
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 82979-6-RR (ARID1B antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human stomach cancer using 82979-6-RR
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 82979-6-RR (ARID1B antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse adrenal gland using 82979-6-RR
Immunohistochemical analysis of paraffin-embedded mouse adrenal gland tissue slide using 82979-6-RR (ARID1B antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse cerebellum using 82979-6-RR
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 82979-6-RR (ARID1B antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat cerebellum using 82979-6-RR
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 82979-6-RR (ARID1B antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HeLa using 82979-6-RR
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ARID1B antibody (82979-6-RR, Clone: 230222A11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).CL594-Phalloidin (red).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
FC experiment of SH-SY5Y using 82979-6-RR
1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug ARID1B Recombinant antibody (82979-6-RR, Clone:230222A11) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Affinity and Kinetic Characterization of 82979-6-RR
Biolayer interferometry (BLl) kinetic assays of 82979-6-RR against Human ARID1B were performed. The affinity constant is 1.14 nM.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive IHC detected in	mouse adrenal gland tissue, human placenta tissue, human stomach cancer tissue, mouse cerebellum tissue, rat cerebellum tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells
Positive FC (Intra) detected in	SH-SY5Y cells

Recommended dilution

Application	Dilution
Immunohistochemistry (IHC)	IHC : 1:8000-1:32000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82979-6-RR targets ARID1B in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag30643 Product name: Recombinant human ARID1B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1370-1508 aa of NM_017519 Sequence: PAKRHEGDMYNMQYSSQQQEMYNQYGGSYSGPDRRPIQGQYPYPYSRERMQGPGQIQTHGIPPQMMGGPLQSSSSEGPQQNMWAARNDMPYPYQNRQGPGGPTQAPPYPGMNRTDDMMVPDQRINHESQWPSHVSQRQP Predict reactive species
Full Name	AT rich interactive domain 1B (SWI1-like)
Calculated Molecular Weight	236 kDa
GenBank Accession Number	NM_017519
Gene Symbol	ARID1B
Gene ID (NCBI)	57492
RRID	AB_3670728
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q8NFD5
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
FC protocol for ARID1B antibody 82979-6-RR	Download protocol
IF protocol for ARID1B antibody 82979-6-RR	Download protocol
IHC protocol for ARID1B antibody 82979-6-RR	Download protocol