|Positive WB detected in||Jurkat cells, human testis tissue|
|Positive IP detected in||Jurkat cells|
|Positive IHC detected in||human brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
15794-1-AP targets ATF6B in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ATF6B fusion protein Ag8502|
|Full Name||activating transcription factor 6 beta|
|Calculated molecular weight||703 aa, 77 kDa|
|Observed molecular weight||110 kDa|
|GenBank accession number||BC008394|
|Gene ID (NCBI)||1388|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The endoplasmic reticulum (ER)-transmembrane proteins, ATF6 alpha (ATF6) and ATF6 beta (ATF6B), are cleaved during the ER stress response (ERSR). The resulting N-terminal fragments of both ATF6 isoforms, which have conserved basic leucine-zipper and DNA binding domains but divergent transcriptional activation domains, translocate to the nucleus where they bind to ER stress-response elements (ERSE) in ERSR, such as Bip/GRP78. This antibody could recognize ATF6 beta (110kd) and its cleaved forms(60,80kd )(PMID: 11256944)
|Product Specific Protocols|
|WB protocol for ATF6B antibody 15794-1-AP||Download protocol|
|IHC protocol for ATF6B antibody 15794-1-AP||Download protocol|
|IF protocol for ATF6B antibody 15794-1-AP||Download protocol|
|IP protocol for ATF6B antibody 15794-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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