• Featured Product
  • KD/KO Validated

ATG7 Polyclonal antibody

ATG7 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (6)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10088-2-AP

Synonyms

APG7 LIKE, APG7L, ATG7, Autophagy related protein 7, GSA7, hAGP7



Tested Applications

Positive WB detected inJurkat cells, THP-1 cells
Positive IHC detected inhuman cervical cancer tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10088-2-AP targets ATG7 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity Chinese soft-shelled turtles, human, mouse, pig, prawns, rat, swine, turtle
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATG7 fusion protein Ag0142
Full Name ATG7 autophagy related 7 homolog (S. cerevisiae)
Calculated molecular weight 78 kDa
Observed molecular weight 68-78 kDa
GenBank accession numberBC000091
Gene symbol ATG7
Gene ID (NCBI) 10533
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Atg7 is an E1-like enzyme that is specifically involved in autophagosome formation and is essential for autophagy. As an autophagic-related protein it is required for linking to Atg12, Atg5 and Atg8, which are essential for Atg conjugation and autophagosome formation. Atg7 has been reported as an important regulator of autophagy with starvation-induced or chemotherapeutic agent treatment. The high expression level of ATG7 is related to the survival of patients with breast cancer. There are several isoforms of ATG7 protein ranged from 68 kDa to 78 kDa.

Protocols

Product Specific Protocols
WB protocol for ATG7 antibody 10088-2-APDownload protocol
IHC protocol for ATG7 antibody 10088-2-APDownload protocol
IF protocol for ATG7 antibody 10088-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
prawnsWB

Ecotoxicol Environ Saf

Evaluating expression of autophagy-related genes in oriental river prawn Macrobrachium nipponense as potential biomarkers for hypoxia exposure.

Authors - Shengming Sun
turtleIHC

Reprod Biol Endocrinol

LIPOPHAGY: a novel form of steroidogenic activity within the LEYDIG cell during the reproductive cycle of turtle.

Authors - Imran Tarique
ratWB

Cell Death Dis

Regulatory roles of miR-22/Redd1-mediated mitochondrial ROS and cellular autophagy in ionizing radiation-induced BMSC injury.

Authors - Zhonglong Liu
mouseWB

Biochem Biophys Res Commun

Autophagy exerts pivotal roles in regulatory effects of 1α,25-(OH)2D3 on the osteoclastogenesis.

Authors - Lianmei Ji
Chinese soft-shelled turtlesIF

Biomolecules

In Vivo Autophagy Up-Regulation of Small Intestine Enterocytes in Chinese Soft-Shelled Turtles during Hibernation.

Authors - Waseem Ali Vistro
humanIHC

Br J Oral Maxillofac Surg

Increased expression of autophagy-related proteins in keratocystic odontogenic tumours: its possible association with growth potential.

Authors - Rui-Fang Li