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WB analysis using 10088-2-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10088-2-AP (ATG7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis using 10088-2-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10088-2-AP (ATG7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
IP experiment of HT-1080 using 10088-2-AP
IP result of anti-ATG7 (IP:10088-2-AP, 4ug; Detection:10088-2-AP 1:500) with HT-1080 cells lysate 3600 ug.
IP experiment of NIH/3T3 using 10088-2-AP
IP result of anti-ATG7 (IP:10088-2-AP, 4ug; Detection:10088-2-AP 1:500) with NIH/3T3 cells lysate 1600 ug.
IHC staining of human cervical cancer using 10088-2-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10088-2-AP (ATG7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human cervical cancer using 10088-2-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10088-2-AP (ATG7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 10088-2-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10088-2-AP (ATG7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 10088-2-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10088-2-AP (ATG7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 10088-2-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ATG7 antibody (10088-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

ATG7 Polyclonal antibody

ATG7 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10088-2-AP

Publications(146)

Reviews (5)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (7)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

GSA7, Autophagy-related protein 7, Autophagy related protein 7, ATG12-activating enzyme E1 ATG7, APG7L

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (146)
Protocols
Reviews (5)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, NIH/3T3 cells, mouse liver tissue, THP-1 cells, U2OS cells
Positive IP detected in	HT-1080 cells, NIH/3T3 cells
Positive IHC detected in	human cervical cancer tissue, mouse kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 21 publications below
WB	See 132 publications below
IHC	See 16 publications below
IF	See 13 publications below
IP	See 1 publications below
CoIP	See 1 publications below

Product Information

10088-2-AP targets ATG7 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat, pig, chicken, hamster, goat, turtle, prawn
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ATG7 fusion protein Ag0142 Predict reactive species
Full Name	ATG7 autophagy related 7 homolog (S. cerevisiae)
Calculated Molecular Weight	78 kDa
Observed Molecular Weight	68-78 kDa
GenBank Accession Number	BC000091
Gene Symbol	ATG7
Gene ID (NCBI)	10533
RRID	AB_2062351
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O95352
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Atg7 is an E1-like enzyme that is specifically involved in autophagosome formation and is essential for autophagy. As an autophagic-related protein it is required for linking to Atg12, Atg5 and Atg8, which are essential for Atg conjugation and autophagosome formation. Atg7 has been reported as an important regulator of autophagy with starvation-induced or chemotherapeutic agent treatment. The high expression level of ATG7 is related to the survival of patients with breast cancer. There are several isoforms of ATG7 protein ranged from 68 kDa to 78 kDa.

Protocols

Product Specific Protocols
WB protocol for ATG7 antibody 10088-2-AP	Download protocol
IHC protocol for ATG7 antibody 10088-2-AP	Download protocol
IF protocol for ATG7 antibody 10088-2-AP	Download protocol
IP protocol for ATG7 antibody 10088-2-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Adv Sci (Weinh) Inhibition of CK2/ING4 Pathway Facilitates Non-Small Cell Lung Cancer Immunotherapy Authors - Qian Gou View Article
human	WB	Nat Commun Inhibition of the CDK2 and Cyclin A complex leads to autophagic degradation of CDK2 in cancer cells. Authors - Jiawei Zhang KD Validated View Article
human	WB	Cell Death Differ STING directly activates autophagy to tune the innate immune response. Authors - Dong Liu View Article
mouse	WB, IHC	Autophagy Autophagy regulates functional differentiation of mammary epithelial cells. Authors - Jessica Elswood View Article
rat	WB	Autophagy Negative pressure wound therapy improves bone regeneration by promoting osteogenic differentiation via the AMPK-ULK1-autophagy axis. Authors - Sheng Zhang View Article
human,mouse	WB	Autophagy AP2M1 mediates autophagy-induced CLDN2 (claudin 2) degradation through endocytosis and interaction with LC3 and reduces intestinal epithelial tight junction permeability. Authors - Ashwinkumar Subramenium Ganapathy KO Validated View Article

Reviews

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The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH Margaux (Verified Customer) (06-20-2025)	Used at 1/1000 in HeLa cells treated or not with siRNA targeting ATG7. I noticed non-specific band and couldn't see the downregulation of ATG7 protein expression in the siRNA condition. Applications: Western Blot
FH Priya (Verified Customer) (10-03-2024)	Used this antibody for colon carcinoma cell line and mice tissue Applications: Western Blot Primary Antibody Dilution: 1 to 1000 Cell Tissue Type: Caco2, Mice colon
FH Priya (Verified Customer) (08-09-2023)	Used for Caco2 cells and mice colon tissue Applications: Western Blot Primary Antibody Dilution: 1 to 1000 Cell Tissue Type: Caco2, Mice colon
FH Kamal (Verified Customer) (06-29-2023)	Atg7 band showed up better than from other companies. Applications: Western Blot Primary Antibody Dilution: 1:2000 Cell Tissue Type: Mouse Liver tissue ATG7 Antibody Western Blot validation (1:2000 dilution) in Mouse Liver tissue (Cat no:10088-2-AP)
FH Priya (Verified Customer) (06-21-2023)	Used this antibody for Caco2 cells and mice tissue Applications: Western Blot Primary Antibody Dilution: 1 to 1000 Cell Tissue Type: Caco2, Mice colon