|Positive WB detected in||Jurkat cells, THP-1 cells|
|Positive IHC detected in||human cervical cancer tissue, mouse kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10088-2-AP targets ATG7 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||Chinese soft-shelled turtles, human, mouse, pig, prawns, rat, swine, turtle|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ATG7 fusion protein Ag0142|
|Full Name||ATG7 autophagy related 7 homolog (S. cerevisiae)|
|Calculated molecular weight||78 kDa|
|Observed molecular weight||68-78 kDa|
|GenBank accession number||BC000091|
|Gene ID (NCBI)||10533|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Atg7 is an E1-like enzyme that is specifically involved in autophagosome formation and is essential for autophagy. As an autophagic-related protein it is required for linking to Atg12, Atg5 and Atg8, which are essential for Atg conjugation and autophagosome formation. Atg7 has been reported as an important regulator of autophagy with starvation-induced or chemotherapeutic agent treatment. The high expression level of ATG7 is related to the survival of patients with breast cancer. There are several isoforms of ATG7 protein ranged from 68 kDa to 78 kDa.
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