ATX3,ATXN3 Monoclonal antibody

ATX3,ATXN3 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1F7E10

Cat no : 67057-1-Ig

Synonyms

AT3, ataxin 3, ATX3, ATXN3, JOS, MJD, MJD1, SCA3



Tested Applications

Positive WB detected inMCF-7 cells, HeLa cells, Jurkat cells, HEK-293 cells, pig brain tissue, mouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Sample-dependent, check data in validation data gallery

Product Information

67057-1-Ig targets ATX3,ATXN3 in WB, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ATX3,ATXN3 fusion protein Ag6627
Full Name ataxin 3
Calculated molecular weight 370 aa, 43 kDa
Observed molecular weight 41-45 kDa
GenBank accession numberBC033711
Gene symbol ATXN3
Gene ID (NCBI) 4287
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

ATXN3, which has deubiquitinase activity and act as a component of the ubiquitin proteasome system, plays a role in transcriptional regulation and neuroprotection. ATXN3 interacts with RAD23, HHR23A and HHR23B, involves in the pathology of MJD. ATXN3 is a mixed-linkage, chain-editing enzyme and that the UIM region of ATXN3 regulates its substrate specificity. Contains an N-terminal deubiquitinating domain, called the Josephin domain, followed by 2 ubiquitin-interacting motifs (UIMs) and a polyQ tract near the C terminus. ATXN3 can be phosphorylated in a protein casein kinase-2-dependent manner, thus the MW would be larger than the predicted one.

Protocols

Product Specific Protocols
WB protocol for ATX3,ATXN3 antibody 67057-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols