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  • Western Blot (WB) analysis of various lysates using Alkyl-DHAP synthase/AGPS Recombinant antibody (86472-3-RR)

    WB analysis using 86472-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86472-3-RR (AGPS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Alkyl-DHAP synthase/AGPS Recombinant antibody (86472-3-RR)

    IF Staining of HeLa using 86472-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Alkyl-DHAP synthase/AGPS antibody (86472-3-RR, Clone: 251340H3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 86472-3-RR

    Affinity and Kinetic Characterization of 86472-3-RR

    Biolayer interferometry (BLl) kinetic assays of 86472-3-RR against Human Alkyl-DHAP synthase/AGPS were performed. The affinity constant is below 1 pM.

Alkyl-DHAP synthase/AGPS Recombinant monoclonal antibody

Alkyl-DHAP synthase/AGPS Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 86472-3-RR
Clone No.251340H3

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

AGPS, AAG5, ADAP S, ADAS, ADHAPS

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

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Tested Applications

Positive WB detected inHeLa cells, MDA-MB-231 cells, SW480 cells, Jurkat cells, THP-1 cells, NIH/3T3 cells, HSC-T6 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86472-3-RR targets Alkyl-DHAP synthase/AGPS in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag15233

Product name: Recombinant human AGPS protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 309-658 aa of BC141820

Sequence: TVGGWVSTRASGMKKNIYGNIEDLVVHIKMVTPRGIIEKSCQGPRMSTGPDIHHFIMGSEGTLGVITEATIKIRPVPEYQKYGSVAFPNFEQGVACLREIAKQRCAPASIRLMDNKQFQFGHALKPQVSSIFTSFLDGLKKFYITKFKGFDPNQLSVATLLFEGDREKVLQHEKQVYDIAAKFGGLAAGEDNGQRGYLLTYVIAYIRDLALEYYVLGESFETSAPWDRVVDLCRNVKERITRECKEKGVQFAPFSTCRVTQTYDAGACIYFYFAFNYRGISDPLTVFEQTEAAAREEILANGGSLSHHHGVGKLRKQWLKESISDVGFGMLKSVKEYVDPNNIFGNRNLL

Predict reactive species
Full Name alkylglycerone phosphate synthase
Calculated Molecular Weight 658 aa, 73 kDa
Observed Molecular Weight 68-70 kDa
GenBank Accession NumberBC141820
Gene Symbol AGPS
Gene ID (NCBI) 8540
Conjugate Unconjugated
FormLiquid
Purification MethodProtein A purification
UNIPROT IDO00116
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for Alkyl-DHAP synthase/AGPS antibody 86472-3-RRDownload protocol
IF protocol for Alkyl-DHAP synthase/AGPS antibody 86472-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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