Beta-2-microglobulin Antibody

Beta-2-microglobulin Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

FC, IF, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat no : 13511-1-AP

Synonyms

B2M, Beta-2-microglobulin, HDCMA22P



Tested Applications

Positive WB detected inA431 cells, HeLa cells, human heart tissue, human stomach tissue, mouse lung tissue, Raji cells, HepG2 cells, Jurkat cells
Positive IP detected inA431 cells
Positive IHC detected inhuman oesophagus cancer tissue, mouse lung tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

13511-1-AP targets Beta-2-microglobulin in FC, IF, IHC, IP, WB, ELISA applications and shows reactivity with human, mouse samples.

Reactivity human, mouse
Cited Species rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Beta-2-microglobulin fusion protein Ag4433
Full Name beta-2-microglobulin
Calculated molecular weight 119aa,14 kDa
Observed molecular weight 12 kDa
GenBank accession number BC032589
Gene symbol B2M
Gene ID (NCBI) 567
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Aliquoting is unnecessary for -20oC storage.

Background Information

Beta-2-microglobulin (B2M) is a component of MHC class I molecules, which are present on the surface of nearly all nucleated cells. It can be found in body fluids under physiologic conditions as a result of shedding from cell surfaces or intracellular release. B2M has various biological functions, including antigen presentation. Investigations reveal that increased synthesis and release of B2M are present in several malignant diseases.

Protocols

Product Specific Protocols
WB protocol for Beta-2-microglobulin antibody 13511-1-APDownload protocol
IHC protocol for Beta-2-microglobulin antibody 13511-1-APDownload protocol
IF protocol for Beta-2-microglobulin antibody 13511-1-APDownload protocol
IP protocol for Beta-2-microglobulin antibody 13511-1-APDownload protocol
FC protocol for Beta-2-microglobulin antibody 13511-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

PLoS One

Heritable Multiplex Genetic Engineering in Rats Using CRISPR/Cas9.

Authors - Yuanwu Ma

Nat Commun

Targeting Tyro3 ameliorates a model of PGRN-mutant FTLD-TDP via tau-mediated synaptic pathology.

Authors - Kyota Fujita