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BACE2 Polyclonal antibody

BACE2 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16321-1-AP

Synonyms

AEPLC, ALP56, Asp 1, ASP1, ASP21, Aspartic like protease 56 kDa, Aspartyl protease 1, BACE2, BAE2, Beta secretase 2, CDA13, CEAP1, Down region aspartic protease, DRAP, Memapsin 1, Theta secretase, UNQ418/PRO852

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using BACE2 Polyclonal antibody (16321-1-AP)

    WB analysis using 16321-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16321-1-AP (BACE2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using BACE2 Polyclonal antibody (16321-1-AP)

    IHC staining of human colon cancer using 16321-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16321-1-AP (BACE2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using BACE2 Polyclonal antibody (16321-1-AP)

    IHC staining of human colon cancer using 16321-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16321-1-AP (BACE2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using BACE2 Polyclonal antibody (16321-1-AP)

    IHC staining of human breast cancer using 16321-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16321-1-AP (BACE2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using BACE2 Polyclonal antibody (16321-1-AP)

    IHC staining of human breast cancer using 16321-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16321-1-AP (BACE2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHEK-293 cells, Hela cells, SH-SY5Y cells
Positive IHC detected inhuman colon cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

16321-1-AP targets BACE2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BACE2 fusion protein Ag9404
Full Name beta-site APP-cleaving enzyme 2
Calculated Molecular Weight 518 aa, 56 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC014453
Gene Symbol BACE2
Gene ID (NCBI) 25825
RRIDAB_2061667
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for BACE2 antibody 16321-1-APDownload protocol
IHC protocol for BACE2 antibody 16321-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Brain Behav Immun

Elevated β-secretase 1 expression mediates CD4+ T cell dysfunction via PGE2 signalling in Alzheimer's disease.

Authors - Linbin Dai
View Article
mouseWB

Int J Neurosci

Activating transcription factor 6 reduces Aβ1-42 and restores memory in Alzheimer's disease model mice.

Authors - Yayun Du
View Article