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BACH1 Monoclonal antibody

BACH1 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2D12A4

Cat no : 66762-1-Ig

Synonyms

BACH 1, BACH1, BTB and CNC homolog 1, HA2303



Tested Applications

Positive WB detected inHEK-293 cells, Jurkat cells, Raji cells, HSC-T6 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66762-1-Ig targets BACH1 in WB, IHC, ELISA applications and shows reactivity with Human, Rat samples.

Tested Reactivity Human, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen BACH1 fusion protein Ag6011
Full Name BTB and CNC homology 1, basic leucine zipper transcription factor 1
Calculated molecular weight 82 kDa
Observed molecular weight 100-110 kDa, 82 kDa
GenBank accession numberBC063307
Gene symbol BACH1
Gene ID (NCBI) 571
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BTB and CNC homology 1, basic leucine zipper transcription factor 1(BACH1) is a heme-regulated transcriptional regulator that acts as repressor or activator and a member of the AP-a superfamily. MafK belongs to the small Maf family that can funciton as transcriptional activators or repressors depending on the proteins with which they form heterdimers. BACH1 has crucial roles in both stress responses and transformation by H-RasV12. The molecular weight of expected mobility Bach1 is 100-110 kDa.

Protocols

Product Specific Protocols
WB protocol for BACH1 antibody 66762-1-IgDownload protocol
IHC protocol for BACH1 antibody 66762-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols