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Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, LNCaP cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83371-1-RR targets BAP1 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag34415 Product name: Recombinant human BAP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 575-729 aa of BC001596 Sequence: LTEGGKGSSPSIRPIQGSQGSSSPVEKEVVEATDSREKTGMVRPGEPLSGEKYSPKELLALLKCVEAEIANYEACLKEEVEKRKKFKIDDQRRTHNYDEFICTFISMLAQEGMLANLVEQNISVRRRQGVSIGRLHKQRKPDRRKRSRPYKAKRQ Predict reactive species |
| Full Name | BRCA1 associated protein-1 (ubiquitin carboxy-terminal hydrolase) |
| Calculated Molecular Weight | 81-91 kDa |
| Observed Molecular Weight | 91-100 kDa |
| GenBank Accession Number | BC001596 |
| Gene Symbol | BAP1 |
| Gene ID (NCBI) | 8314 |
| RRID | AB_3671025 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q92560 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for BAP1 antibody 83371-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
