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BAP29 Polyclonal antibody

BAP29 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 15796-1-AP

Synonyms

BAP29, BCAP29

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BAP29 Polyclonal antibody (15796-1-AP)

    WB analysis using 15796-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15796-1-AP (BAP29 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using BAP29 Polyclonal antibody (15796-1-AP)

    WB analysis of COLO 320 using 15796-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 15796-1-AP (BAP29 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using BAP29 Polyclonal antibody (15796-1-AP)

    IHC staining of human lymphoma using 15796-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 15796-1-AP (BAP29 antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using BAP29 Polyclonal antibody (15796-1-AP)

    IHC staining of human lymphoma using 15796-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 15796-1-AP (BAP29 antibody) at dilution of 1:200 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using BAP29 Polyclonal antibody (15796-1-AP)

    IF Staining of HeLa using 15796-1-AP

    Immunofluorescent analysis of Hela cells, using BCAP29 antibody 15796-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Flow cytometry (FC) experiment of HEK-293 cells using BAP29 Polyclonal antibody (15796-1-AP)

    FC experiment of HEK-293 using 15796-1-AP

    1X10^6 HEK-293 cells were stained with 0.2ug BAP29 antibody (15796-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). FITC-Goat anti-Rabbit IgG with dilution 1:100.

Tested Applications

Positive WB detected inJurkat cells, COLO 320 cells, Raji cells, U-87 MG cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells
Positive FC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15796-1-AP targets BAP29 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BAP29 fusion protein Ag8505
Full Name B-cell receptor-associated protein 29
Calculated Molecular Weight 241 aa, 28 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC008478
Gene Symbol BCAP29
Gene ID (NCBI) 55973
RRIDAB_2065118
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for BAP29 antibody 15796-1-APDownload protocol
IHC protocol for BAP29 antibody 15796-1-APDownload protocol
IF protocol for BAP29 antibody 15796-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Xin (Verified Customer) (03-19-2021)

The protein ran slightly lower than the predicted size (28kDa), it was detected somewhere around 24kDa.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: A549 cells
BAP29 Antibody Western Blot validation (1:1000 dilution) in A549 cells (Cat no:15796-1-AP)